Effects of Temperature on In Vitro Short-Term Storage of Sterlet Sturgeon (Acipenser Ruthenus) Ova

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F16%3A43890369" target="_blank" >RIV/60076658:12520/16:43890369 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/rda.12661/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/rda.12661/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/rda.12661" target="_blank" >10.1111/rda.12661</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of Temperature on In Vitro Short-Term Storage of Sterlet Sturgeon (Acipenser Ruthenus) Ova

Popis výsledku v původním jazyce

Artificial propagation of sturgeons is becoming increasingly important for recovery efforts as well as for commercial production. Sterlet Acipenser ruthenus is a common Eurasian sturgeon with a small body size and one of the fastest reproductive cycles among the sturgeons. The practical question being addressed in this study was how long fertilization of ovulated eggs can be delayed without substantially reducing the hatching rate, and an ancillary question is under what' temperature conditions do eggs retain good quality. Brood-stock were injected with homogenized carp pituitary extract (CPE); ovulated eggs from three females were allocated to various treatment groups for temperature storage (control, 7 degrees C, 11 degrees C, 15 degrees C and 19 degrees C) until fertilized. Storage times at the regulated temperatures prior to fertilization were for 2.5, 5.0, 7.5 and 10.0 h. After the selected storage times in ovarian fluid, eggs were fertilized and transferred to incubation cages and then they were counted. Three replicates were allocated to each storage period and temperature. Hatched larvae were counted at 7-day post-fertilization. We found that sterlet eggs do not need to be fertilized immediately after collection. Reasonably good quality was retained for several hours if temperature conditions are fairly cool and stable. Eggs retained good quality when stored at 7 degrees C and 11 degrees C for up to 10 h with 54.1 +/- 2.9 to 69.9 +/- 7.9% hatching success, but egg quality was significantly reduced after 5-h storage at 19 degrees C (p < 0.01) and 7.5-h storage at 15 degrees C (p < 0.05) compared to cooler temperatures. Uniform temperatures between 7 degrees C and 11 degrees C can be considered as appropriate for storage of eggs in ovarian fluid for up to 10 h. This information can have practical application to routine hatchery practice for acipenserids, as well as for certain research protocols.

Název v anglickém jazyce

Effects of Temperature on In Vitro Short-Term Storage of Sterlet Sturgeon (Acipenser Ruthenus) Ova

Popis výsledku anglicky

Artificial propagation of sturgeons is becoming increasingly important for recovery efforts as well as for commercial production. Sterlet Acipenser ruthenus is a common Eurasian sturgeon with a small body size and one of the fastest reproductive cycles among the sturgeons. The practical question being addressed in this study was how long fertilization of ovulated eggs can be delayed without substantially reducing the hatching rate, and an ancillary question is under what' temperature conditions do eggs retain good quality. Brood-stock were injected with homogenized carp pituitary extract (CPE); ovulated eggs from three females were allocated to various treatment groups for temperature storage (control, 7 degrees C, 11 degrees C, 15 degrees C and 19 degrees C) until fertilized. Storage times at the regulated temperatures prior to fertilization were for 2.5, 5.0, 7.5 and 10.0 h. After the selected storage times in ovarian fluid, eggs were fertilized and transferred to incubation cages and then they were counted. Three replicates were allocated to each storage period and temperature. Hatched larvae were counted at 7-day post-fertilization. We found that sterlet eggs do not need to be fertilized immediately after collection. Reasonably good quality was retained for several hours if temperature conditions are fairly cool and stable. Eggs retained good quality when stored at 7 degrees C and 11 degrees C for up to 10 h with 54.1 +/- 2.9 to 69.9 +/- 7.9% hatching success, but egg quality was significantly reduced after 5-h storage at 19 degrees C (p < 0.01) and 7.5-h storage at 15 degrees C (p < 0.05) compared to cooler temperatures. Uniform temperatures between 7 degrees C and 11 degrees C can be considered as appropriate for storage of eggs in ovarian fluid for up to 10 h. This information can have practical application to routine hatchery practice for acipenserids, as well as for certain research protocols.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Reproduction in Domestic Animals

ISSN

0936-6768

e-ISSN

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Svazek periodika

51

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

165-170

Kód UT WoS článku

000373107600023

EID výsledku v databázi Scopus

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