Level of in vitro storage of the European catfish (Silurus glanis L.) eggs at different temperatures

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902496" target="_blank" >RIV/60076658:12520/21:43902496 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/s10695-020-00902-9" target="_blank" >https://doi.org/10.1007/s10695-020-00902-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10695-020-00902-9" target="_blank" >10.1007/s10695-020-00902-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Level of in vitro storage of the European catfish (Silurus glanis L.) eggs at different temperatures

Popis výsledku v původním jazyce

European catfish (Silurus glanis) is a commercially important freshwater fish originating from Eastern Europe. The objective of this study was to examine the short-term storage of its eggs especially in relation to maintaining a low level of malformation in newly hatched fry. The eggs from freshly spawned individuals were stored separately in cell incubators at 17 and 22 degrees C under aerobic conditions. Changes in fertilization, hatching, and malformation were examined in eggs stored at 1, 3, 5, and 7 h post-stripping. The sperm used for fertilization showed very good motility rates (84-90%) and curvilinear velocity (110-125 mu m/s), and straight-line velocity did not drop below 77 mu m/s. For all females, a temperature of 17 degrees C was better than 22 degrees C for egg storage in vitro. Egg fertilization and total hatching decreased rapidly after 7 h storage at 17 degrees C. The storage time of eggs in vitro to fertilization should therefore not exceed 5 h at 17 degrees C. In all females, there was no difference in the total number of eggs hatching between 1 and 3 h of egg storage at 17 degrees C. The storage time of eggs did not correlate with the level of malformations of the fry. However, the level of hatching and malformations was clearly affected by the storage temperature of eggs when it was &gt; 17 degrees C. Analysis showed that the storage time of eggs, temperature of storage, and individual females had a significant influence on fertilization and total hatching rates. Regression analysis confirmed a low correlation of fertilization and hatching rates with storage time of eggs.

Název v anglickém jazyce

Level of in vitro storage of the European catfish (Silurus glanis L.) eggs at different temperatures

Popis výsledku anglicky

European catfish (Silurus glanis) is a commercially important freshwater fish originating from Eastern Europe. The objective of this study was to examine the short-term storage of its eggs especially in relation to maintaining a low level of malformation in newly hatched fry. The eggs from freshly spawned individuals were stored separately in cell incubators at 17 and 22 degrees C under aerobic conditions. Changes in fertilization, hatching, and malformation were examined in eggs stored at 1, 3, 5, and 7 h post-stripping. The sperm used for fertilization showed very good motility rates (84-90%) and curvilinear velocity (110-125 mu m/s), and straight-line velocity did not drop below 77 mu m/s. For all females, a temperature of 17 degrees C was better than 22 degrees C for egg storage in vitro. Egg fertilization and total hatching decreased rapidly after 7 h storage at 17 degrees C. The storage time of eggs in vitro to fertilization should therefore not exceed 5 h at 17 degrees C. In all females, there was no difference in the total number of eggs hatching between 1 and 3 h of egg storage at 17 degrees C. The storage time of eggs did not correlate with the level of malformations of the fry. However, the level of hatching and malformations was clearly affected by the storage temperature of eggs when it was &gt; 17 degrees C. Analysis showed that the storage time of eggs, temperature of storage, and individual females had a significant influence on fertilization and total hatching rates. Regression analysis confirmed a low correlation of fertilization and hatching rates with storage time of eggs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fish Physiology and Biochemistry

ISSN

0920-1742

e-ISSN

—

Svazek periodika

47

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

163-171

Kód UT WoS článku

000593040000001

EID výsledku v databázi Scopus

2-s2.0-85096515700