Sperm and egg in vitro storage effects on artificial fertilization and hatching in common carp (Cyprinus carpio L.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F23%3A43906227" target="_blank" >RIV/60076658:12520/23:43906227 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.aqrep.2023.101507" target="_blank" >https://doi.org/10.1016/j.aqrep.2023.101507</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aqrep.2023.101507" target="_blank" >10.1016/j.aqrep.2023.101507</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sperm and egg in vitro storage effects on artificial fertilization and hatching in common carp (Cyprinus carpio L.)

Popis výsledku v původním jazyce

This is the first report showing fertilization of short-term stored eggs (19 degrees C) with short-term stored diluted sperm (0-2 degrees C) in common carp (Cyprinus carpio). From 5 males, fresh (control) and 7-day stored sperm were diluted with common carp extender. After collection of eggs from nine females, the eggs were immediately pooled into three groups from females 1-3, 4-6 and 7-9, according to the eggs&apos; quality based on visual criteria. They were placed in beakers, covered and incubated at 19 degrees C (for each pool, 10 g of eggs from each female were used). The 7-day short-term storage sperm was incubated at 20 degrees C for 20 min and pooled before being used for fertilization. The eggs were fertilized with fresh and short-term storage sperm after 1, 3 and 6 h post storage of eggs and activated with hatchery water and Perchec solution. Sperm motility and velocity (50-54% and 80-115 mu m/s) of 7-day short-term storage sperm at 0-2 degrees C were ensured by additional incubation of sperm at 20 degrees C for 20 min before being activated. Principal component analysis showed that the sorting of eggs of different females into three groups according to sperm quality, fertilization ability and production of malformed larvae was successful. It is recommended that overripe common carp eggs are stored for a maximum of 1 h. A period of 3 h can be used for better quality eggs and exceptionally, up to 6 h for very high-quality eggs. Higher larvae malformation was found in the 6 h aged eggs together with fertilized short-term storage and fresh sperm. In all quality groups of eggs, the effect of different sperm, activation medium and egg storage time on the level of malformations was observed. The activation solution for carp did not show any positive fertilization and hatching effects.

Název v anglickém jazyce

Sperm and egg in vitro storage effects on artificial fertilization and hatching in common carp (Cyprinus carpio L.)

Popis výsledku anglicky

This is the first report showing fertilization of short-term stored eggs (19 degrees C) with short-term stored diluted sperm (0-2 degrees C) in common carp (Cyprinus carpio). From 5 males, fresh (control) and 7-day stored sperm were diluted with common carp extender. After collection of eggs from nine females, the eggs were immediately pooled into three groups from females 1-3, 4-6 and 7-9, according to the eggs&apos; quality based on visual criteria. They were placed in beakers, covered and incubated at 19 degrees C (for each pool, 10 g of eggs from each female were used). The 7-day short-term storage sperm was incubated at 20 degrees C for 20 min and pooled before being used for fertilization. The eggs were fertilized with fresh and short-term storage sperm after 1, 3 and 6 h post storage of eggs and activated with hatchery water and Perchec solution. Sperm motility and velocity (50-54% and 80-115 mu m/s) of 7-day short-term storage sperm at 0-2 degrees C were ensured by additional incubation of sperm at 20 degrees C for 20 min before being activated. Principal component analysis showed that the sorting of eggs of different females into three groups according to sperm quality, fertilization ability and production of malformed larvae was successful. It is recommended that overripe common carp eggs are stored for a maximum of 1 h. A period of 3 h can be used for better quality eggs and exceptionally, up to 6 h for very high-quality eggs. Higher larvae malformation was found in the 6 h aged eggs together with fertilized short-term storage and fresh sperm. In all quality groups of eggs, the effect of different sperm, activation medium and egg storage time on the level of malformations was observed. The activation solution for carp did not show any positive fertilization and hatching effects.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture Reports

ISSN

2352-5134

e-ISSN

—

Svazek periodika

29

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

—

Kód UT WoS článku

000944819500001

EID výsledku v databázi Scopus

2-s2.0-85149863987