The plasma membrane contributes to temperature-induced spermatozoa motility kinetics in short-term stored sperm of common carp (Cyprinus carpio)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F24%3A43908134" target="_blank" >RIV/60076658:12520/24:43908134 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.aquaculture.2024.740588" target="_blank" >https://doi.org/10.1016/j.aquaculture.2024.740588</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2024.740588" target="_blank" >10.1016/j.aquaculture.2024.740588</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The plasma membrane contributes to temperature-induced spermatozoa motility kinetics in short-term stored sperm of common carp (Cyprinus carpio)

Popis výsledku v původním jazyce

Spermatozoa motility kinetics have been found to increase in short-term stored sperm on ice of common carp (Cyprinus carpio) following incubation at a high temperature (20 degrees C) for 10-20 min. The aim of the present study was to elucidate the contribution of the ATP store and plasma membrane function in temperature -induced motility kinetics of short-term stored sperm. Spermatozoa ATP content significantly decreased after 9 days of short-term storage on ice; however, it remained unchanged after incubating stored sperm at higher temperatures. Spermatozoa motility kinetics of either fresh or stored sperm on ice for 14 days were enhanced following incubation or activation at 20 degrees C. The motility of demembranated spermatozoa from 14 days of stored sperm could be nearly fully activated, similar to that of fresh spermatozoa. The swelling ratio of spermatozoa incubated and activated at high temperatures was higher than that at low temperatures. Spermatozoa swelling was observed to be faster from sperm stored for 14 days than fresh sperm at the same incubation and activation temperature. These results suggest a physiological contribution of the plasma membrane to enhance spermatozoa motility kinetics in stored sperm following incubation at 20 degrees C.

Název v anglickém jazyce

The plasma membrane contributes to temperature-induced spermatozoa motility kinetics in short-term stored sperm of common carp (Cyprinus carpio)

Popis výsledku anglicky

Spermatozoa motility kinetics have been found to increase in short-term stored sperm on ice of common carp (Cyprinus carpio) following incubation at a high temperature (20 degrees C) for 10-20 min. The aim of the present study was to elucidate the contribution of the ATP store and plasma membrane function in temperature -induced motility kinetics of short-term stored sperm. Spermatozoa ATP content significantly decreased after 9 days of short-term storage on ice; however, it remained unchanged after incubating stored sperm at higher temperatures. Spermatozoa motility kinetics of either fresh or stored sperm on ice for 14 days were enhanced following incubation or activation at 20 degrees C. The motility of demembranated spermatozoa from 14 days of stored sperm could be nearly fully activated, similar to that of fresh spermatozoa. The swelling ratio of spermatozoa incubated and activated at high temperatures was higher than that at low temperatures. Spermatozoa swelling was observed to be faster from sperm stored for 14 days than fresh sperm at the same incubation and activation temperature. These results suggest a physiological contribution of the plasma membrane to enhance spermatozoa motility kinetics in stored sperm following incubation at 20 degrees C.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

1873-5622

Svazek periodika

583

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

—

Kód UT WoS článku

001170462600001

EID výsledku v databázi Scopus

2-s2.0-85183511518