AQUA_2020_1080: Sperm management of European catfish (Silurus glanis L.) for effective reproduction and genetic conservation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43901013" target="_blank" >RIV/60076658:12520/20:43901013 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.aquaculture.2020.735620" target="_blank" >https://doi.org/10.1016/j.aquaculture.2020.735620</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2020.735620" target="_blank" >10.1016/j.aquaculture.2020.735620</a>

Jazyk výsledku

angličtina

Název v původním jazyce

AQUA_2020_1080: Sperm management of European catfish (Silurus glanis L.) for effective reproduction and genetic conservation

Popis výsledku v původním jazyce

The European catfish (Silurus glanis; also called wels or sheatfish) is one of the largest commercially important freshwater fish species. The objective of this study was to examine the storage time of its semen in an immobilization solution (IS) to enhance conservation and aquaculture programs. Changes in spermatozoan motility was examined in semen stored on ice in 20 mL tubes in IS for 0, 1, 7, 14 and 28 days post-stripping (DPS). Stored sperm samples were pooled and used for fertility testing at 1 and 7 DPS. The IS maintained sperm motility at &gt; 90% during days 1-7 of semen storage, and curvilinear velocity (VCL) and straight-line velocity (VSL) were up to the levels of 163 and 90 mu m s(-1), respectively. Spermatozoa stored for 14 days had 60% motility and VCL and VSL values were similar to those of 7 DPS. Sperm motility was only 1% at 28 DPS with VCL and VSL of 79 and 24 mu m s(-1), respectively. The fertilization and total hatching rates of eggs activated with sperm stored for 7 days were reduced, but the slight decrease was compensated by the increase in supply of spermatozoa which resulted in overall 80% level of fertility and hatching. The number of larval malformations was not correlated with storage time of sperm. Based on the results of this study, sperm from the European catfish can be collected and stored in IS for as long as 1 week, retaining quality sufficient for fertilization to yield normal fry.

Název v anglickém jazyce

AQUA_2020_1080: Sperm management of European catfish (Silurus glanis L.) for effective reproduction and genetic conservation

Popis výsledku anglicky

The European catfish (Silurus glanis; also called wels or sheatfish) is one of the largest commercially important freshwater fish species. The objective of this study was to examine the storage time of its semen in an immobilization solution (IS) to enhance conservation and aquaculture programs. Changes in spermatozoan motility was examined in semen stored on ice in 20 mL tubes in IS for 0, 1, 7, 14 and 28 days post-stripping (DPS). Stored sperm samples were pooled and used for fertility testing at 1 and 7 DPS. The IS maintained sperm motility at &gt; 90% during days 1-7 of semen storage, and curvilinear velocity (VCL) and straight-line velocity (VSL) were up to the levels of 163 and 90 mu m s(-1), respectively. Spermatozoa stored for 14 days had 60% motility and VCL and VSL values were similar to those of 7 DPS. Sperm motility was only 1% at 28 DPS with VCL and VSL of 79 and 24 mu m s(-1), respectively. The fertilization and total hatching rates of eggs activated with sperm stored for 7 days were reduced, but the slight decrease was compensated by the increase in supply of spermatozoa which resulted in overall 80% level of fertility and hatching. The number of larval malformations was not correlated with storage time of sperm. Based on the results of this study, sperm from the European catfish can be collected and stored in IS for as long as 1 week, retaining quality sufficient for fertilization to yield normal fry.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

—

Svazek periodika

529

Číslo periodika v rámci svazku

neuveden

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

—

Kód UT WoS článku

000566468000001

EID výsledku v databázi Scopus

2-s2.0-85086874445