Survivability of Boar Sperm Diluted in Long-Term Extender Containing Kofola

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F21%3AN0000262" target="_blank" >RIV/00027014:_____/21:N0000262 - isvavai.cz</a>

Výsledek na webu

<a href="http://vuzv.cz" target="_blank" >http://vuzv.cz</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Survivability of Boar Sperm Diluted in Long-Term Extender Containing Kofola

Popis výsledku v původním jazyce

The objective of this study was to evaluate the survivability of boar sperm diluted in long-term extender containing kofola. Eight sperm rich fractions from 4 fertile boars from one AI centre with motility ≥ 80% and the number of morphologically abnormal spermatozoa ≤ 25% were used in this study. Kofola original – KO was added to boar semen extender Androstar plus (AS+). Semen was diluted in AS+ as a control (K) and AS+ with KO in the dilution ratio 1+2. Samples were stored at 17°C and evaluated after 0h, 48h, 92h and 168h storage time for sperm motility and sperm viability. SCA system was used for determined sperm motility (total, progressive, non-progressive and immotile) and kinetic characteristics - straight line velocity (VSL, μm/s), average path velocity (VAP, μm/s), curvilinear velocity (VCL, μm/s), straightness (STR, VSL/VAP, %), linearity (LIN, VSL/VCL, %), wobble (WOB, VAP/VCL, %). Statistically significant differences (P < 0.05) were recorded between K (94.47, 73.91, 5.53%) vs. KO (87.76, 61.10, 12.24%) in total mean values of total sperm motility, progressive and immotile sperm. The progressive sperm motility was 13% lower in KO than K at 96h storage time then showed a sharp decrease after 168 h. The sperm survival was significantly affected by storage time (P < 0.05). Between kinetic indicator of speed not reported significant differences between K vs. KO (P > 0.05). No difference between K (77.75%) vs. KO (79.44) were found in percentage of sperm viability (P > 0.05). In conclusion; the results of motility and viability point to the possibility of using kofola original as a potential ingredient in long- term boar extender until the storage time of 96 hours.

Název v anglickém jazyce

Survivability of Boar Sperm Diluted in Long-Term Extender Containing Kofola

Popis výsledku anglicky

The objective of this study was to evaluate the survivability of boar sperm diluted in long-term extender containing kofola. Eight sperm rich fractions from 4 fertile boars from one AI centre with motility ≥ 80% and the number of morphologically abnormal spermatozoa ≤ 25% were used in this study. Kofola original – KO was added to boar semen extender Androstar plus (AS+). Semen was diluted in AS+ as a control (K) and AS+ with KO in the dilution ratio 1+2. Samples were stored at 17°C and evaluated after 0h, 48h, 92h and 168h storage time for sperm motility and sperm viability. SCA system was used for determined sperm motility (total, progressive, non-progressive and immotile) and kinetic characteristics - straight line velocity (VSL, μm/s), average path velocity (VAP, μm/s), curvilinear velocity (VCL, μm/s), straightness (STR, VSL/VAP, %), linearity (LIN, VSL/VCL, %), wobble (WOB, VAP/VCL, %). Statistically significant differences (P < 0.05) were recorded between K (94.47, 73.91, 5.53%) vs. KO (87.76, 61.10, 12.24%) in total mean values of total sperm motility, progressive and immotile sperm. The progressive sperm motility was 13% lower in KO than K at 96h storage time then showed a sharp decrease after 168 h. The sperm survival was significantly affected by storage time (P < 0.05). Between kinetic indicator of speed not reported significant differences between K vs. KO (P > 0.05). No difference between K (77.75%) vs. KO (79.44) were found in percentage of sperm viability (P > 0.05). In conclusion; the results of motility and viability point to the possibility of using kofola original as a potential ingredient in long- term boar extender until the storage time of 96 hours.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

40203 - Husbandry

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Research in Pig Breeding

ISSN

1802-7547

e-ISSN

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Svazek periodika

15

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

6-11

Kód UT WoS článku

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EID výsledku v databázi Scopus

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