Sperm Motility and Viability in Boar Sperm Diluted in Long-Term Extender Containing Different Type of Kofola

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F21%3AN0000267" target="_blank" >RIV/00027014:_____/21:N0000267 - isvavai.cz</a>

Výsledek na webu

<a href="https://vuzv.cz/wp-content/uploads/2022/01/21258.pdf" target="_blank" >https://vuzv.cz/wp-content/uploads/2022/01/21258.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Sperm Motility and Viability in Boar Sperm Diluted in Long-Term Extender Containing Different Type of Kofola

Popis výsledku v původním jazyce

The objective of this study was to evaluate the sperm motility and viability of boar sperm diluted in long-term extender containing different types of kofola. Eight sperm rich fractions from 4 fertile boars from one AI centre with motility ≥80% and the number of morphologically abnormal spermatozoa ≤ 25% were used in this study. Tested types of kofola: mix of spices – KS (cinnamon extract 100mg/l and clove extract 100mg/l) and honey – KH (honey extract 100mg/l) were added to boar semen extender Androstar plus (AS+). Semen was diluted in AS+ as a control (K) and AS+ with addition of different types of kofola in the dilution ratio 1+2. Samples were stored at 17°C and evaluated after 0h, 48h, 92h and 168h storage time for sperm motility and sperm viability. SCA system was used for determined sperm motility (total, progressive, non-progressive and immotile) and kinetic characteristics - straight line velocity (VSL, μm/s), average path velocity (VAP, μm/s), curvilinear velocity (VCL, μm/s), straightness (STR, %), linearity (LIN, %), wobble (WOB, %). Statistically significant differences in total mean values of total sperm motility, progressive and immotile sperm (P<0.05) were recorded between K (94.47, 73.91, 5.53%) vs. KH (85.58, 59.85, 14.43%) and KS (87.10, 60.96, 12.91%). The sperm survival was significantly affected by storage time (P<0.05). Kinetic indicator of speed VCL and LIN reported significant differences between K (57.73 μm/s, 36.16%) vs. KH (49.10 μm/s, 39.52%) P<0.05. No differences were found in percentage of sperm viability (P>0.05) between K (77.75%) vs. KH (77.88%) and KS (79.81%). In conclusion, application of tested types of kofola as a potential component to the boar extender unfortunately did not have a positive effect on boar sperm motility, but the sperm viability was not adversely affected.

Název v anglickém jazyce

Sperm Motility and Viability in Boar Sperm Diluted in Long-Term Extender Containing Different Type of Kofola

Popis výsledku anglicky

The objective of this study was to evaluate the sperm motility and viability of boar sperm diluted in long-term extender containing different types of kofola. Eight sperm rich fractions from 4 fertile boars from one AI centre with motility ≥80% and the number of morphologically abnormal spermatozoa ≤ 25% were used in this study. Tested types of kofola: mix of spices – KS (cinnamon extract 100mg/l and clove extract 100mg/l) and honey – KH (honey extract 100mg/l) were added to boar semen extender Androstar plus (AS+). Semen was diluted in AS+ as a control (K) and AS+ with addition of different types of kofola in the dilution ratio 1+2. Samples were stored at 17°C and evaluated after 0h, 48h, 92h and 168h storage time for sperm motility and sperm viability. SCA system was used for determined sperm motility (total, progressive, non-progressive and immotile) and kinetic characteristics - straight line velocity (VSL, μm/s), average path velocity (VAP, μm/s), curvilinear velocity (VCL, μm/s), straightness (STR, %), linearity (LIN, %), wobble (WOB, %). Statistically significant differences in total mean values of total sperm motility, progressive and immotile sperm (P<0.05) were recorded between K (94.47, 73.91, 5.53%) vs. KH (85.58, 59.85, 14.43%) and KS (87.10, 60.96, 12.91%). The sperm survival was significantly affected by storage time (P<0.05). Kinetic indicator of speed VCL and LIN reported significant differences between K (57.73 μm/s, 36.16%) vs. KH (49.10 μm/s, 39.52%) P<0.05. No differences were found in percentage of sperm viability (P>0.05) between K (77.75%) vs. KH (77.88%) and KS (79.81%). In conclusion, application of tested types of kofola as a potential component to the boar extender unfortunately did not have a positive effect on boar sperm motility, but the sperm viability was not adversely affected.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

40203 - Husbandry

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Research in Pig Breeding

ISSN

1802-7547

e-ISSN

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Svazek periodika

15

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

1-6

Kód UT WoS článku

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EID výsledku v databázi Scopus

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