A novel method for rapid elimination of sturgeon egg stickiness using sodium hypochlorite

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F16%3A43890370" target="_blank" >RIV/60076658:12520/16:43890370 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0044848615302672" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0044848615302672</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2015.11.039" target="_blank" >10.1016/j.aquaculture.2015.11.039</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A novel method for rapid elimination of sturgeon egg stickiness using sodium hypochlorite

Popis výsledku v původním jazyce

Reducing egg stickiness is a crucial step in artificial culture of sturgeon eggs, with commonly used methods being time consuming or interfering with hatching. Sodium hypochlorite (SH) at varying concentrations and exposure times was tested on sterlet Acipenser ruthenus eggs, with 0.03% SH for 40 s effectively eliminating stickiness without impairment to eggs, embryo development, or hatching when compared with other de-adhesion methods (clay; NaCl, urea, and tannic acid). Field tests using sterlet, Siberian sturgeon Acipenser baerii, and Russian sturgeon Acipenser gueldenstaedtii showed similar numbers of larvae hatched using SH and clay. Immunohistochemistry revealed a protein carbonyl group only on the surface of eggs treated with SH, evidence of oxidation as the mode of action and indicating that oxidation did not affect inner egg layers or cytoplasm. Treatment with SH is a rapid, simple, and inexpensive method for de-adhesion of sturgeon eggs. Statement of relevance: Sturgeons represent a valuable group of fish in aquaculture, especially for caviar, but also for meat production. This study describes a novel approach to sturgeon egg de-adhesion involving oxidation of egg surface. The method requires little time, is low cost, and disinfects and softens the egg surface, which facilitates hatching.

Název v anglickém jazyce

A novel method for rapid elimination of sturgeon egg stickiness using sodium hypochlorite

Popis výsledku anglicky

Reducing egg stickiness is a crucial step in artificial culture of sturgeon eggs, with commonly used methods being time consuming or interfering with hatching. Sodium hypochlorite (SH) at varying concentrations and exposure times was tested on sterlet Acipenser ruthenus eggs, with 0.03% SH for 40 s effectively eliminating stickiness without impairment to eggs, embryo development, or hatching when compared with other de-adhesion methods (clay; NaCl, urea, and tannic acid). Field tests using sterlet, Siberian sturgeon Acipenser baerii, and Russian sturgeon Acipenser gueldenstaedtii showed similar numbers of larvae hatched using SH and clay. Immunohistochemistry revealed a protein carbonyl group only on the surface of eggs treated with SH, evidence of oxidation as the mode of action and indicating that oxidation did not affect inner egg layers or cytoplasm. Treatment with SH is a rapid, simple, and inexpensive method for de-adhesion of sturgeon eggs. Statement of relevance: Sturgeons represent a valuable group of fish in aquaculture, especially for caviar, but also for meat production. This study describes a novel approach to sturgeon egg de-adhesion involving oxidation of egg surface. The method requires little time, is low cost, and disinfects and softens the egg surface, which facilitates hatching.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

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Svazek periodika

453

Číslo periodika v rámci svazku

Neuveden

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

73-76

Kód UT WoS článku

000366803800009

EID výsledku v databázi Scopus

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