The second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F16%3A43890372" target="_blank" >RIV/60076658:12520/16:43890372 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985904:_____/16:00458511

Výsledek na webu

<a href="http://gsejournal.biomedcentral.com/articles/10.1186/s12711-016-0194-0" target="_blank" >http://gsejournal.biomedcentral.com/articles/10.1186/s12711-016-0194-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12711-016-0194-0" target="_blank" >10.1186/s12711-016-0194-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity

Popis výsledku v původním jazyce

Background: One of the five basal actinopterygian lineages, the Chondrostei, including sturgeon, shovelnose, and paddlefish (Order Acipenseriformes) show extraordinary ploidy diversity associated with three rounds of lineage-specific whole-genome duplication, resulting in three levels of ploidy in sturgeon. Recently, incidence of spontaneous polyploidization has been reported among cultured sturgeon and it could have serious negative implications for the economics of sturgeon farming. We report the occurrence of seven spontaneous heptaploid (7n) Siberian sturgeon Acipenser baerii, which is a functional tetraploid species (4n) with similar to 245 chromosomes. Our aims were to assess ploidy level and chromosome number of the analysed specimens and to identify the possible mechanism that underlies the occurrence of spontaneous additional chromosome sets in their genome. Results: Among 150 specimens resulting from the mating of a tetraploid (4n) A. baerii (similar to 245 chromosomes) dam with a hexaploid (6n) A. baerii (similar to 368 chromosomes) sire, 143 displayed a relative DNA content that corresponds to pentaploidy (5n) with an absolute DNA content of 8.98 +/- 0.03 pg DNA per nucleus and nuclear area of 35.3 +/- 4.3 mu m(2) and seven specimens exhibited a relative DNA content that corresponds to heptaploidy (7n), with an absolute DNA content of 15.02 +/- 0.04 pg DNA per nucleus and nuclear area of 48.4 +/- 5.1 mu m(2). Chromosome analyses confirmed a modal number of similar to 437 chromosomes in these heptaploid (7n) individuals. DNA genotyping of eight microsatellite loci followed by parental assignment confirmed spontaneous duplication of the maternal chromosome sets via retention of the second polar body in meiosis II as the mechanism for the formation of this unusual chromosome number and ploidy level in a functional tetraploid A. baerii.

Název v anglickém jazyce

The second highest chromosome count among vertebrates is observed in cultured sturgeon and is associated with genome plasticity

Popis výsledku anglicky

Background: One of the five basal actinopterygian lineages, the Chondrostei, including sturgeon, shovelnose, and paddlefish (Order Acipenseriformes) show extraordinary ploidy diversity associated with three rounds of lineage-specific whole-genome duplication, resulting in three levels of ploidy in sturgeon. Recently, incidence of spontaneous polyploidization has been reported among cultured sturgeon and it could have serious negative implications for the economics of sturgeon farming. We report the occurrence of seven spontaneous heptaploid (7n) Siberian sturgeon Acipenser baerii, which is a functional tetraploid species (4n) with similar to 245 chromosomes. Our aims were to assess ploidy level and chromosome number of the analysed specimens and to identify the possible mechanism that underlies the occurrence of spontaneous additional chromosome sets in their genome. Results: Among 150 specimens resulting from the mating of a tetraploid (4n) A. baerii (similar to 245 chromosomes) dam with a hexaploid (6n) A. baerii (similar to 368 chromosomes) sire, 143 displayed a relative DNA content that corresponds to pentaploidy (5n) with an absolute DNA content of 8.98 +/- 0.03 pg DNA per nucleus and nuclear area of 35.3 +/- 4.3 mu m(2) and seven specimens exhibited a relative DNA content that corresponds to heptaploidy (7n), with an absolute DNA content of 15.02 +/- 0.04 pg DNA per nucleus and nuclear area of 48.4 +/- 5.1 mu m(2). Chromosome analyses confirmed a modal number of similar to 437 chromosomes in these heptaploid (7n) individuals. DNA genotyping of eight microsatellite loci followed by parental assignment confirmed spontaneous duplication of the maternal chromosome sets via retention of the second polar body in meiosis II as the mechanism for the formation of this unusual chromosome number and ploidy level in a functional tetraploid A. baerii.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Genetics Selection Evolution

ISSN

0999-193X

e-ISSN

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Svazek periodika

48

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000369815500001

EID výsledku v databázi Scopus

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