The effects of GnRHa with and without dopamine antagonist on reproductive hormone levels and ovum viability in tench Tinca tinca

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F16%3A43890537" target="_blank" >RIV/60076658:12520/16:43890537 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0044848616304677" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0044848616304677</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2016.09.012" target="_blank" >10.1016/j.aquaculture.2016.09.012</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effects of GnRHa with and without dopamine antagonist on reproductive hormone levels and ovum viability in tench Tinca tinca

Popis výsledku v původním jazyce

The study evaluated the impact of mGnRHa ([D-Tle(6), Pro(9), NEt]-mGnRH), with or without metoclopramide, on release of luteinizing hormone (LH) and sex steroids and on viability of ova in tench Tinca tinca. Four experimental groups were subjected to the following treatments given as intraperitoneal injection: 0.9% NaCl (C); 25 mu g kg(-1) mGnRHa (Gn); 25 mu g kg(-1) mGnRHa with 20 mg kg(-1) metoclopramide (GnM); and or 20 mg kg(-1) metoclopramide (M). GnM treatment induced LH surge reaching maximum concentrations 6 h post-treatment followed by a slight but significant decline (P < 0.05). Gn group showed gradually increasing LH values (P < 0.05). No significant differences were found between GnM and Gn treated groups in 17 beta-estradiol (E-2) or testosterone blood level. Significant decrease from baseline (P < 0.05) was found in E-2 values at the end of trial period in both Gn and GnM. Testosterone values increased with Gn and the GnM, peaking 12 h post-treatment and dropping to baseline levels at 24 h. GnM was associated with significant increase of 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one (17,20 beta-P) values at 6 h (P < 0.05), with its concentration peaking at 12 h similar to that in Gn. Both treatments induced ovulation in a high proportion of females, while M and C group showed no ovulation. No significant differences were found in ovulation rate, latency, ovum weight, or relative fecundity. Nevertheless, significant differences (P < 0.05) between Gn and GnM treatment were observed in fertility (77% vs. 54%) and hatching (40% vs. 23%). GnM stimulated rapid LH release with a potentiating effect on 17,20 beta-P secretion, possibly the source of the adverse effects on ovum viability. We describe for the first time the negative impact of metoclopramide combined with mGnRHa hormone treatment on ovum quality in tench. Statement of relevance: Improvement of fish artificial reproduction.

Název v anglickém jazyce

The effects of GnRHa with and without dopamine antagonist on reproductive hormone levels and ovum viability in tench Tinca tinca

Popis výsledku anglicky

The study evaluated the impact of mGnRHa ([D-Tle(6), Pro(9), NEt]-mGnRH), with or without metoclopramide, on release of luteinizing hormone (LH) and sex steroids and on viability of ova in tench Tinca tinca. Four experimental groups were subjected to the following treatments given as intraperitoneal injection: 0.9% NaCl (C); 25 mu g kg(-1) mGnRHa (Gn); 25 mu g kg(-1) mGnRHa with 20 mg kg(-1) metoclopramide (GnM); and or 20 mg kg(-1) metoclopramide (M). GnM treatment induced LH surge reaching maximum concentrations 6 h post-treatment followed by a slight but significant decline (P < 0.05). Gn group showed gradually increasing LH values (P < 0.05). No significant differences were found between GnM and Gn treated groups in 17 beta-estradiol (E-2) or testosterone blood level. Significant decrease from baseline (P < 0.05) was found in E-2 values at the end of trial period in both Gn and GnM. Testosterone values increased with Gn and the GnM, peaking 12 h post-treatment and dropping to baseline levels at 24 h. GnM was associated with significant increase of 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one (17,20 beta-P) values at 6 h (P < 0.05), with its concentration peaking at 12 h similar to that in Gn. Both treatments induced ovulation in a high proportion of females, while M and C group showed no ovulation. No significant differences were found in ovulation rate, latency, ovum weight, or relative fecundity. Nevertheless, significant differences (P < 0.05) between Gn and GnM treatment were observed in fertility (77% vs. 54%) and hatching (40% vs. 23%). GnM stimulated rapid LH release with a potentiating effect on 17,20 beta-P secretion, possibly the source of the adverse effects on ovum viability. We describe for the first time the negative impact of metoclopramide combined with mGnRHa hormone treatment on ovum quality in tench. Statement of relevance: Improvement of fish artificial reproduction.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GL - Rybářství

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

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Svazek periodika

465

Číslo periodika v rámci svazku

December

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

158-163

Kód UT WoS článku

000385372200022

EID výsledku v databázi Scopus

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