The effect of GnRHa with or without dopamine inhibitor on reproductive hormone levels and sperm quality in tench Tinca tinca

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F17%3A43892108" target="_blank" >RIV/60076658:12520/17:43892108 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0044848616305282" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0044848616305282</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2016.12.023" target="_blank" >10.1016/j.aquaculture.2016.12.023</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of GnRHa with or without dopamine inhibitor on reproductive hormone levels and sperm quality in tench Tinca tinca

Popis výsledku v původním jazyce

The impact of treatment with mGnRHa, mGnRHa with metoclopramide, and metoclopramide on secretion of luteinizing hormone (LH), testosterone (T) and 11-ketotestosterone (11-KT) and on milt and spermatozoon characteristics was studied in male tench Tinca tinca. Male tench were divided into four groups and treated with following preparations: 0.9% NaCI; mGnRHa (25 mu g kg(-1)); mGnRHa (25 mu g kg(-1)) combined with metoclopramide (20 mg kg(-1)); and metoclopramide (20 mg kg(-1)). Administration of the combined treatment induced an LH surge at 12 h post injection (PI) reaching significantly higher values.(P &lt; 0.05) than was observed with stimulation by mGnRHa alone or with metoclopramide alone. Monitoring of LH levels demonstrated a rapid surge in LH release with the combined treatment versus gradual increase with mGnRHa alone (P &lt; 0.05). Experimental treatments with GnRHa alone, metoclopramide alone and metoclopramide combined with mGnRHa significantly increased milt production and relative number of spermatozoa kg(-1) BW at 24 h PI compared to controls, with no differences among experimental groups (P &lt; 0.05). No significant differences among groups (P &lt; 0.05) were found in motility or velocity (VLC, VAP, VSL). The only significant decrease of VLC and VAP was detected after treatment with metoclopramide alone compared to all other groups. Nonsignificant differences (P &lt; 0.05) were observed in T and 11-KT secretion, with the exception of a significant difference in T secretion (P &lt; 0.05) at 24 h PI between the combined group and the metoclopramide treatment and the controls. We conclude that the use of GnRH, combined or not with metoclopramide, are suitable hormonal treatments to control the spermiation in mature tench.

Název v anglickém jazyce

The effect of GnRHa with or without dopamine inhibitor on reproductive hormone levels and sperm quality in tench Tinca tinca

Popis výsledku anglicky

The impact of treatment with mGnRHa, mGnRHa with metoclopramide, and metoclopramide on secretion of luteinizing hormone (LH), testosterone (T) and 11-ketotestosterone (11-KT) and on milt and spermatozoon characteristics was studied in male tench Tinca tinca. Male tench were divided into four groups and treated with following preparations: 0.9% NaCI; mGnRHa (25 mu g kg(-1)); mGnRHa (25 mu g kg(-1)) combined with metoclopramide (20 mg kg(-1)); and metoclopramide (20 mg kg(-1)). Administration of the combined treatment induced an LH surge at 12 h post injection (PI) reaching significantly higher values.(P &lt; 0.05) than was observed with stimulation by mGnRHa alone or with metoclopramide alone. Monitoring of LH levels demonstrated a rapid surge in LH release with the combined treatment versus gradual increase with mGnRHa alone (P &lt; 0.05). Experimental treatments with GnRHa alone, metoclopramide alone and metoclopramide combined with mGnRHa significantly increased milt production and relative number of spermatozoa kg(-1) BW at 24 h PI compared to controls, with no differences among experimental groups (P &lt; 0.05). No significant differences among groups (P &lt; 0.05) were found in motility or velocity (VLC, VAP, VSL). The only significant decrease of VLC and VAP was detected after treatment with metoclopramide alone compared to all other groups. Nonsignificant differences (P &lt; 0.05) were observed in T and 11-KT secretion, with the exception of a significant difference in T secretion (P &lt; 0.05) at 24 h PI between the combined group and the metoclopramide treatment and the controls. We conclude that the use of GnRH, combined or not with metoclopramide, are suitable hormonal treatments to control the spermiation in mature tench.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

—

Svazek periodika

470

Číslo periodika v rámci svazku

1 March 2017

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

91-94

Kód UT WoS článku

000394197900012

EID výsledku v databázi Scopus

2-s2.0-85007422224