Molecular Characterization of PGC-1 beta (PPAR Gamma Coactivator 1 beta) and its Roles in Mitochondrial Biogenesis in Blunt Snout Bream (Megalobrama amblycephala)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43900894" target="_blank" >RIV/60076658:12520/20:43900894 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.3390/ijms21061935" target="_blank" >https://doi.org/10.3390/ijms21061935</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms21061935" target="_blank" >10.3390/ijms21061935</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular Characterization of PGC-1 beta (PPAR Gamma Coactivator 1 beta) and its Roles in Mitochondrial Biogenesis in Blunt Snout Bream (Megalobrama amblycephala)

Popis výsledku v původním jazyce

This study aimed at achieving the molecular characterization of peroxisome proliferator-activated receptor-gamma coactivator 1 beta (PGC-1 beta) and exploring its modulatory roles in mitochondria biogenesis in blunt snout bream (Megalobrama amblycephala). A full-length cDNA of PGC-1 beta was cloned from liver which covered 3110 bp encoding 859 amino acids. The conserved motifs of PGC-1 beta family proteins were gained by MEME software, and the phylogenetic analyses showed motif loss and rearrangement of PGC-1 beta in fish. The function of PGC-1 beta was evaluated through overexpression and knockdown of PGC-1 beta in primary hepatocytes of blunt snout bream. We observed overexpression of PGC-1 beta along with enhanced mitochondrial transcription factor A (TFAM) expression and mtDNA copies in hepatocytes, and its knockdown led to slightly reduced NRF1 expression. However, knockdown of PGC-1 beta did not significantly influence TFAM expression or mtDNA copies. The alterations in mitochondria biogenesis were assessed following high-fat intake, and the results showed that it induces downregulation of PGC-1 beta. Furthermore, significant decreases in mitochondrial respiratory chain activities and mitochondria biogenesis were observed by high-fat intake. Our findings demonstrated that overexpression of PGC-1 beta induces the enhancement of TFAM expression and mtDNA amount but not NRF-1. Therefore, it could be concluded that PGC-1 beta is involved in mitochondrial biogenesis in blunt snout bream but not through PGC-1 beta/NRF-1 pathway.

Název v anglickém jazyce

Molecular Characterization of PGC-1 beta (PPAR Gamma Coactivator 1 beta) and its Roles in Mitochondrial Biogenesis in Blunt Snout Bream (Megalobrama amblycephala)

Popis výsledku anglicky

This study aimed at achieving the molecular characterization of peroxisome proliferator-activated receptor-gamma coactivator 1 beta (PGC-1 beta) and exploring its modulatory roles in mitochondria biogenesis in blunt snout bream (Megalobrama amblycephala). A full-length cDNA of PGC-1 beta was cloned from liver which covered 3110 bp encoding 859 amino acids. The conserved motifs of PGC-1 beta family proteins were gained by MEME software, and the phylogenetic analyses showed motif loss and rearrangement of PGC-1 beta in fish. The function of PGC-1 beta was evaluated through overexpression and knockdown of PGC-1 beta in primary hepatocytes of blunt snout bream. We observed overexpression of PGC-1 beta along with enhanced mitochondrial transcription factor A (TFAM) expression and mtDNA copies in hepatocytes, and its knockdown led to slightly reduced NRF1 expression. However, knockdown of PGC-1 beta did not significantly influence TFAM expression or mtDNA copies. The alterations in mitochondria biogenesis were assessed following high-fat intake, and the results showed that it induces downregulation of PGC-1 beta. Furthermore, significant decreases in mitochondrial respiratory chain activities and mitochondria biogenesis were observed by high-fat intake. Our findings demonstrated that overexpression of PGC-1 beta induces the enhancement of TFAM expression and mtDNA amount but not NRF-1. Therefore, it could be concluded that PGC-1 beta is involved in mitochondrial biogenesis in blunt snout bream but not through PGC-1 beta/NRF-1 pathway.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

1422-0067

Svazek periodika

21

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

—

Kód UT WoS článku

000529890200031

EID výsledku v databázi Scopus

2-s2.0-85081615701