Standardization of egg activation and fertilization in sterlet (Acipenser ruthenus)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43900933" target="_blank" >RIV/60076658:12520/20:43900933 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.aqrep.2020.100381" target="_blank" >https://doi.org/10.1016/j.aqrep.2020.100381</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aqrep.2020.100381" target="_blank" >10.1016/j.aqrep.2020.100381</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Standardization of egg activation and fertilization in sterlet (Acipenser ruthenus)

Popis výsledku v původním jazyce

This study was conducted to determine the minimum number of spermatozoa and optimal volume of activation water for effective fertilization of sterlet Acipenser nnhenus eggs. It was also to determine the minimum concentration of spermatozoa per ml of activation water during artificial propagation, when sperm with good motility and velocity (95 % motility and 150 mu m s(-1) curvilinear velocity) were used for fertilization. In three experiments, eggs (similar to 50) from three sterlet were fertilized by pooled sperm from four males in four replicates and incubated at 17 degrees C. The success of fertilization was evaluated at neurulation and hatching. Sterlet eggs were fertilized in proportion to the concentration of spermatozoa per ml in activation water. A total of 50,000 spermatozoa in 8 ml or 32 ml of activation water (concentrations of 6,250 and 1,563 spermatozoa per ml of activation water, respectively) was sufficient for successful fertilization based on neurulation and hatching rates. Neurulation and hatching gradually decreased with a decrease of the spermatozoa egg ratio from 6,250 to 114. A ratio 1 divided by 4 between eggs and water was insufficient to provide adequate fertilization. Highest neurulation and hatching ratios were reported when the egg divided by water ratio reached 1 divided by 15, which is recommended for practical usage. Our results therefore showed that a very low concentration of 100 - 1,300 spermatozoa per ml with around 95 % motility and curvilinear velocity over 150 mu m s(-1) in activation water can provide effective fertilization. In studies of fishes with external fertilization, the volume of activation water during fertilization, total number of spermatozoa and eggs and/or concentration of sperm in activation water should be defined.

Název v anglickém jazyce

Standardization of egg activation and fertilization in sterlet (Acipenser ruthenus)

Popis výsledku anglicky

This study was conducted to determine the minimum number of spermatozoa and optimal volume of activation water for effective fertilization of sterlet Acipenser nnhenus eggs. It was also to determine the minimum concentration of spermatozoa per ml of activation water during artificial propagation, when sperm with good motility and velocity (95 % motility and 150 mu m s(-1) curvilinear velocity) were used for fertilization. In three experiments, eggs (similar to 50) from three sterlet were fertilized by pooled sperm from four males in four replicates and incubated at 17 degrees C. The success of fertilization was evaluated at neurulation and hatching. Sterlet eggs were fertilized in proportion to the concentration of spermatozoa per ml in activation water. A total of 50,000 spermatozoa in 8 ml or 32 ml of activation water (concentrations of 6,250 and 1,563 spermatozoa per ml of activation water, respectively) was sufficient for successful fertilization based on neurulation and hatching rates. Neurulation and hatching gradually decreased with a decrease of the spermatozoa egg ratio from 6,250 to 114. A ratio 1 divided by 4 between eggs and water was insufficient to provide adequate fertilization. Highest neurulation and hatching ratios were reported when the egg divided by water ratio reached 1 divided by 15, which is recommended for practical usage. Our results therefore showed that a very low concentration of 100 - 1,300 spermatozoa per ml with around 95 % motility and curvilinear velocity over 150 mu m s(-1) in activation water can provide effective fertilization. In studies of fishes with external fertilization, the volume of activation water during fertilization, total number of spermatozoa and eggs and/or concentration of sperm in activation water should be defined.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture Reports

ISSN

2352-5134

e-ISSN

—

Svazek periodika

17

Číslo periodika v rámci svazku

neuveden

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

—

Kód UT WoS článku

000544610700006

EID výsledku v databázi Scopus

2-s2.0-85085327351