Gamete management for artificial reproduction of northern pike Esox lucius (Linnaeus, 1758)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43900942" target="_blank" >RIV/60076658:12520/20:43900942 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.aquaculture.2020.735575" target="_blank" >https://doi.org/10.1016/j.aquaculture.2020.735575</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2020.735575" target="_blank" >10.1016/j.aquaculture.2020.735575</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Gamete management for artificial reproduction of northern pike Esox lucius (Linnaeus, 1758)

Popis výsledku v původním jazyce

Eyeing and hatching rates with regard to sperm: egg ratio, the length of time eggs remain viable post-activation in water, and duration of storage time in testicular and stripped sperm were determined in northern pike Esox lucius. In a trial of seven sperm: egg ratios, we found 4 x 10(5) to be the minimum tested number of spermatozoa per egg to produce an acceptable hatching rate (66 +/- 4%). To determine the period of time that eggs remain viable after activation, eggs were placed in hatchery water and fertilized at 0, 15, 30, 45, 60, 90, 120, 240, and 480 s post-activation. With elapsed time, the eyeing and hatching rates decreased significantly (P &lt; .05), dropping from 83 +/- 8% and 73 +/- 1% at time 0 to 69 +/- 7% and 62 +/- 1%, respectively, after 30 s. At 480 s post-activation, the eyeing and hatching values were 23 +/- 8% and 15 +/- 4%. Produced eyeing and hatching rates and sperm motility parameters of testicular and stripped sperm were compared over the course of 48 h storage. Testicular sperm remained viable for 24 h compared with 3 h for stripped sperm. The obtained data clarified important biological characteristics and interaction of gametes for use in developing optimal techniques for artificial reproduction of northern pike under controlled conditions.

Název v anglickém jazyce

Gamete management for artificial reproduction of northern pike Esox lucius (Linnaeus, 1758)

Popis výsledku anglicky

Eyeing and hatching rates with regard to sperm: egg ratio, the length of time eggs remain viable post-activation in water, and duration of storage time in testicular and stripped sperm were determined in northern pike Esox lucius. In a trial of seven sperm: egg ratios, we found 4 x 10(5) to be the minimum tested number of spermatozoa per egg to produce an acceptable hatching rate (66 +/- 4%). To determine the period of time that eggs remain viable after activation, eggs were placed in hatchery water and fertilized at 0, 15, 30, 45, 60, 90, 120, 240, and 480 s post-activation. With elapsed time, the eyeing and hatching rates decreased significantly (P &lt; .05), dropping from 83 +/- 8% and 73 +/- 1% at time 0 to 69 +/- 7% and 62 +/- 1%, respectively, after 30 s. At 480 s post-activation, the eyeing and hatching values were 23 +/- 8% and 15 +/- 4%. Produced eyeing and hatching rates and sperm motility parameters of testicular and stripped sperm were compared over the course of 48 h storage. Testicular sperm remained viable for 24 h compared with 3 h for stripped sperm. The obtained data clarified important biological characteristics and interaction of gametes for use in developing optimal techniques for artificial reproduction of northern pike under controlled conditions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

—

Svazek periodika

528

Číslo periodika v rámci svazku

neuveden

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

—

Kód UT WoS článku

000553682600030

EID výsledku v databázi Scopus

2-s2.0-85085881416