Energy pathways associated with sustained spermatozoon motility in the endangered Siberian sturgeonAcipenser baerii

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43901019" target="_blank" >RIV/60076658:12520/20:43901019 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1111/jfb.14382" target="_blank" >https://doi.org/10.1111/jfb.14382</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jfb.14382" target="_blank" >10.1111/jfb.14382</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Energy pathways associated with sustained spermatozoon motility in the endangered Siberian sturgeonAcipenser baerii

Popis výsledku v původním jazyce

Sturgeon spermatozoa are unique for their sustained motility. We investigated the relative importance of bioenergetic pathways in the energy supply of Siberian sturgeonAcipenser baeriispermatozoa during motile and immotile states. Spermatozoon motility and oxygen consumption rate (OCR) were analysed following exposure to inhibitors of oxidative phosphorylation (sodium azide, NaN3), glycolysis (2-deoxy-D-glucose, DOG) and beta-oxidation of fatty acids (sodium fluoride, NaF), and to an uncoupler of oxidative phosphorylation (carbonyl cyanidem-chlorophenyl hydrazine, CCCP). No significant difference in curvilinear velocity was observed after addition of these reagents to activation medium (AM) or nonactivation medium (NAM) for incubation. Incubation of spermatozoa in NAM containing CCCP or NaN(3)resulted in significantly decreased motility duration compared to controls. The OCR of sturgeon spermatozoa in AM (11.9 +/- 1.4 nmol O(2)min(-1)(10(9) spz)(-1)) was significantly higher than in NAM (8.2 +/- 1.5 nmol O(2)min(-1)(10(9) spz)(-1)). The OCR significantly declined with addition of NaN(3)to AM and NAM. No significant difference in motility parameters or OCR was observed with NaF or DOG. These results suggest active oxidative phosphorylation in both immotile and motile spermatozoa. Nevertheless, mitochondrial respiration occurring during motility is not sufficient to meet the high energy demands, and the energy required for sustained motility of Siberian sturgeon spermatozoa is derived from adenosine triphosphate accumulated during the quiescent state.

Název v anglickém jazyce

Energy pathways associated with sustained spermatozoon motility in the endangered Siberian sturgeonAcipenser baerii

Popis výsledku anglicky

Sturgeon spermatozoa are unique for their sustained motility. We investigated the relative importance of bioenergetic pathways in the energy supply of Siberian sturgeonAcipenser baeriispermatozoa during motile and immotile states. Spermatozoon motility and oxygen consumption rate (OCR) were analysed following exposure to inhibitors of oxidative phosphorylation (sodium azide, NaN3), glycolysis (2-deoxy-D-glucose, DOG) and beta-oxidation of fatty acids (sodium fluoride, NaF), and to an uncoupler of oxidative phosphorylation (carbonyl cyanidem-chlorophenyl hydrazine, CCCP). No significant difference in curvilinear velocity was observed after addition of these reagents to activation medium (AM) or nonactivation medium (NAM) for incubation. Incubation of spermatozoa in NAM containing CCCP or NaN(3)resulted in significantly decreased motility duration compared to controls. The OCR of sturgeon spermatozoa in AM (11.9 +/- 1.4 nmol O(2)min(-1)(10(9) spz)(-1)) was significantly higher than in NAM (8.2 +/- 1.5 nmol O(2)min(-1)(10(9) spz)(-1)). The OCR significantly declined with addition of NaN(3)to AM and NAM. No significant difference in motility parameters or OCR was observed with NaF or DOG. These results suggest active oxidative phosphorylation in both immotile and motile spermatozoa. Nevertheless, mitochondrial respiration occurring during motility is not sufficient to meet the high energy demands, and the energy required for sustained motility of Siberian sturgeon spermatozoa is derived from adenosine triphosphate accumulated during the quiescent state.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Fish Biology

ISSN

0022-1112

e-ISSN

1095-8649

Svazek periodika

97

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

435-443

Kód UT WoS článku

000541450100001

EID výsledku v databázi Scopus

2-s2.0-85087170589