First identification of dopamine receptors in pikeperch, Sander lucioperca, during the pre-ovulatory period

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43901152" target="_blank" >RIV/60076658:12520/20:43901152 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.cbd.2020.100747" target="_blank" >https://doi.org/10.1016/j.cbd.2020.100747</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cbd.2020.100747" target="_blank" >10.1016/j.cbd.2020.100747</a>

Jazyk výsledku

angličtina

Název v původním jazyce

First identification of dopamine receptors in pikeperch, Sander lucioperca, during the pre-ovulatory period

Popis výsledku v původním jazyce

Dopamine (DA) is a ubiquitous neurotransmitter exerting a range of pleiotropic actions through two DA receptor families, the D1 and the D2. To date in vertebrates, a maximum of four receptor subtypes have been identified within the D1 family, D-1 (former D-1A), D-5 (former D-1B), D-6 (former D-1C and D-1D) and D-7 (former D-1E), while the D2 family encloses five subtypes, D-2, D-3, D-4, D-8 (former D-2like or D-2l) and D-9 (former D4-related sequence or D4-rs). In teleosts, no study has investigated in parallel all the DA receptors to identify and localize the whole receptor repertoire from both families. In pikeperch, Sander lucioperca, a species of interest for aquaculture development, the existence, number and location of the DA receptors are totally unknown. To address these questions, RNA-seq with de novo transcriptome reconstruction, functional annotation and phylogenetic analysis were performed to characterize the transcript repertoire of DA receptors in the brain of female pikeperch at the pre-ovulatory period. Ten different cDNA were identified and showed to belong to the D-1 family: two D-1, one D-5a, one D-6a and one D-6b and to the D2 family: two spliced variants of D-2, one D-3, one D-8 and one D-9. Unlike zebrafish, the subtypes D-4 and D-7 have not yet been isolated in pikeperch. As expected D-1, D-3, D-8 and D-9 are mostly expressed in brain parts except for the cerebellum (D-1 and D-3). The inter-species differences in the number of DA receptors and the inter-organ differences in the gene expression of all receptors support the complexity of the dopaminergic actions in vertebrate.

Název v anglickém jazyce

First identification of dopamine receptors in pikeperch, Sander lucioperca, during the pre-ovulatory period

Popis výsledku anglicky

Dopamine (DA) is a ubiquitous neurotransmitter exerting a range of pleiotropic actions through two DA receptor families, the D1 and the D2. To date in vertebrates, a maximum of four receptor subtypes have been identified within the D1 family, D-1 (former D-1A), D-5 (former D-1B), D-6 (former D-1C and D-1D) and D-7 (former D-1E), while the D2 family encloses five subtypes, D-2, D-3, D-4, D-8 (former D-2like or D-2l) and D-9 (former D4-related sequence or D4-rs). In teleosts, no study has investigated in parallel all the DA receptors to identify and localize the whole receptor repertoire from both families. In pikeperch, Sander lucioperca, a species of interest for aquaculture development, the existence, number and location of the DA receptors are totally unknown. To address these questions, RNA-seq with de novo transcriptome reconstruction, functional annotation and phylogenetic analysis were performed to characterize the transcript repertoire of DA receptors in the brain of female pikeperch at the pre-ovulatory period. Ten different cDNA were identified and showed to belong to the D-1 family: two D-1, one D-5a, one D-6a and one D-6b and to the D2 family: two spliced variants of D-2, one D-3, one D-8 and one D-9. Unlike zebrafish, the subtypes D-4 and D-7 have not yet been isolated in pikeperch. As expected D-1, D-3, D-8 and D-9 are mostly expressed in brain parts except for the cerebellum (D-1 and D-3). The inter-species differences in the number of DA receptors and the inter-organ differences in the gene expression of all receptors support the complexity of the dopaminergic actions in vertebrate.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Comparative Biochemistry and Physiology D-Genomics &amp; Proteomics

ISSN

1744-117X

e-ISSN

—

Svazek periodika

36

Číslo periodika v rámci svazku

neuveden

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

—

Kód UT WoS článku

000591917000008

EID výsledku v databázi Scopus

2-s2.0-85091766161