D1, but not D2, dopamine receptor regulates steroid levels during the final stages of pikeperch gametogenesis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F18%3A43898152" target="_blank" >RIV/60076658:12520/18:43898152 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.cambridge.org/core/journals/animal/article/d1-but-not-d2-dopamine-receptor-regulates-steroid-levels-during-the-final-stages-of-pikeperch-gametogenesis/63DF51686C65A5368E2E5AC07758A848" target="_blank" >https://www.cambridge.org/core/journals/animal/article/d1-but-not-d2-dopamine-receptor-regulates-steroid-levels-during-the-final-stages-of-pikeperch-gametogenesis/63DF51686C65A5368E2E5AC07758A848</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S1751731118000824" target="_blank" >10.1017/S1751731118000824</a>

Jazyk výsledku

angličtina

Název v původním jazyce

D1, but not D2, dopamine receptor regulates steroid levels during the final stages of pikeperch gametogenesis

Popis výsledku v původním jazyce

In pikeperch, Sander lucioperca, aquaculture hormonal treatment is usually applied to synchronize ovulation. However, the effect of dopamine (DA) receptor antagonists, in particular those blocking the D1 DA receptors, remains unknown. Thus, the aim of the present study was to investigate and compare the effects of D1 and D2 DA receptor antagonists on the sex-steroid production and reproductive performance of the species. Two experiments were performed during which mature pikeperch females were injected with different molecules: NaCl 0.9% (negative control) or human chorionic gonadotropin 500 IU/kg (positive control) in both experiments, metoclopramide (a D2 receptor antagonist 4 mg/kg or 20 mg/kg) or SCH23390 (a D1 receptor antagonist 0.8 mg/kg or 4 mg/kg) alone (experiment 1) or in combination with a salmon gonadotropin-releasing hormone analogue (sGnRHa at 25 mu g/kg; experiment 2). In experiment 2, fish were also injected with sGnRHa (25 mu g/kg) as positive control. Samplings of oocytes and blood were performed on the day of injection and after 24 h (both experiments), after 48 h (experiment 2) and at the time of ovulation (both experiments). In non-ovulating fish, samplings were performed 7 days (experiment 1) or 14 days (experiment 2) after injection. In experiment 2, various zootechnical parameters of fertilized eggs were recorded (survival, hatching and malformation rates). The two antagonists alone were ineffective in inducing the final stages and regulating sex-steroid (testosterone, 11 ketotestosterone, 17 beta estradiol and 17,20 beta-dihydroxy-4-pregnen-3-one) production. When administered with sGnRHa, both SCH23390 and metoclopramide induced the final stages. However, only SCH23390 stimulated testosterone (4 mg/kg) and 17 beta estradiol (0.8 mg/kg) production compared with sGnRHa alone. None of the treatments affected the survival, hatching or malformation rates. This is the first report suggesting that in pikeperch the D1, but not the D2, DA receptor antagonist would be involved in the testosterone and 17 beta estradiol production as a potentiator of the sGnRHa effect.

Název v anglickém jazyce

D1, but not D2, dopamine receptor regulates steroid levels during the final stages of pikeperch gametogenesis

Popis výsledku anglicky

In pikeperch, Sander lucioperca, aquaculture hormonal treatment is usually applied to synchronize ovulation. However, the effect of dopamine (DA) receptor antagonists, in particular those blocking the D1 DA receptors, remains unknown. Thus, the aim of the present study was to investigate and compare the effects of D1 and D2 DA receptor antagonists on the sex-steroid production and reproductive performance of the species. Two experiments were performed during which mature pikeperch females were injected with different molecules: NaCl 0.9% (negative control) or human chorionic gonadotropin 500 IU/kg (positive control) in both experiments, metoclopramide (a D2 receptor antagonist 4 mg/kg or 20 mg/kg) or SCH23390 (a D1 receptor antagonist 0.8 mg/kg or 4 mg/kg) alone (experiment 1) or in combination with a salmon gonadotropin-releasing hormone analogue (sGnRHa at 25 mu g/kg; experiment 2). In experiment 2, fish were also injected with sGnRHa (25 mu g/kg) as positive control. Samplings of oocytes and blood were performed on the day of injection and after 24 h (both experiments), after 48 h (experiment 2) and at the time of ovulation (both experiments). In non-ovulating fish, samplings were performed 7 days (experiment 1) or 14 days (experiment 2) after injection. In experiment 2, various zootechnical parameters of fertilized eggs were recorded (survival, hatching and malformation rates). The two antagonists alone were ineffective in inducing the final stages and regulating sex-steroid (testosterone, 11 ketotestosterone, 17 beta estradiol and 17,20 beta-dihydroxy-4-pregnen-3-one) production. When administered with sGnRHa, both SCH23390 and metoclopramide induced the final stages. However, only SCH23390 stimulated testosterone (4 mg/kg) and 17 beta estradiol (0.8 mg/kg) production compared with sGnRHa alone. None of the treatments affected the survival, hatching or malformation rates. This is the first report suggesting that in pikeperch the D1, but not the D2, DA receptor antagonist would be involved in the testosterone and 17 beta estradiol production as a potentiator of the sGnRHa effect.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animal

ISSN

1751-7311

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

2587-2597

Kód UT WoS článku

000450622800017

EID výsledku v databázi Scopus

2-s2.0-85045732741