Spectroscopic Approach to Correction and Visualisation of Bright-Field Light Transmission Microscopy Biological Data

Kód výsledku v IS VaVaI

RIV/60076658:12520/21:43902708 - isvavai.cz

Nalezeny alternativní kódy

RIV/00216208:11320/21:10436149

Výsledek na webu

https://doi.org/10.3390/photonics8080333

DOI - Digital Object Identifier

10.3390/photonics8080333

Jazyk výsledku

angličtina

Název v původním jazyce

Spectroscopic Approach to Correction and Visualisation of Bright-Field Light Transmission Microscopy Biological Data

Popis výsledku v původním jazyce

The most realistic information about a transparent sample such as a live cell can be obtained using bright-field light microscopy. Under high-intensity pulsing LED illumination, we captured a primary 12-bit-per-channel (bpc) response from an observed sample using a bright-field microscope equipped with a high-resolution (4872 x 3248) image sensor. In order to suppress data distortions originating from the light interactions with elements in the optical path, poor sensor reproduction (geometrical defects of the camera sensor and some peculiarities of sensor sensitivity), we propose a spectroscopic approach for the correction of these uncompressed 12 bpc data by simultaneous calibration of all parts of the experimental arrangement. Moreover, the final intensities of the corrected images are proportional to the photon fluxes detected by a camera sensor. It can be visualized in 8 bpc intensity depth after the Least Information Loss compression.

Název v anglickém jazyce

Spectroscopic Approach to Correction and Visualisation of Bright-Field Light Transmission Microscopy Biological Data

Popis výsledku anglicky

The most realistic information about a transparent sample such as a live cell can be obtained using bright-field light microscopy. Under high-intensity pulsing LED illumination, we captured a primary 12-bit-per-channel (bpc) response from an observed sample using a bright-field microscope equipped with a high-resolution (4872 x 3248) image sensor. In order to suppress data distortions originating from the light interactions with elements in the optical path, poor sensor reproduction (geometrical defects of the camera sensor and some peculiarities of sensor sensitivity), we propose a spectroscopic approach for the correction of these uncompressed 12 bpc data by simultaneous calibration of all parts of the experimental arrangement. Moreover, the final intensities of the corrected images are proportional to the photon fluxes detected by a camera sensor. It can be visualized in 8 bpc intensity depth after the Least Information Loss compression.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

LM2018099: Jihočeské výzkumné centrum akvakultury a biodiverzity hydrocenóz

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Photonics

ISSN

2304-6732

e-ISSN

2304-6732

Svazek periodika

8

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

18

Strana od-do

—

Kód UT WoS článku

000690188000001

EID výsledku v databázi Scopus

2-s2.0-85113770395