Optimization of sterlet sperm concentration for cryopreservation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43904058" target="_blank" >RIV/60076658:12520/21:43904058 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.aquaculture.2021.736682" target="_blank" >https://doi.org/10.1016/j.aquaculture.2021.736682</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2021.736682" target="_blank" >10.1016/j.aquaculture.2021.736682</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Optimization of sterlet sperm concentration for cryopreservation

Popis výsledku v původním jazyce

One of the critical points in low temperature storage is the expense of storage capacities. Moreover, sperm concentration in a sample is not only a parameter for effective use of a cryobanks but also a parameter affecting cryopreservation outcomes. Sturgeon sperm in comparison to other species is significantly less concentrated due to the specificity of sperm maturation process, during which sperm is mixed with urine. In this study, we evaluated whether the artificial modification of the sterlet spermatozoa concentration before freezing can be useful during the routine application of sturgeon sperm cryopreservation. The sperm was first concentrated by centrifugation of native sperm samples. Then, the excess of seminal fluid was collected. The concentrated sperm samples were further diluted with the same seminal fluid to obtain different sperm concentrations in suspension, and they were subjected to freeze-thawing. The percentage of postthaw sperm motility depended on the sperm concentration in the samples. The highest post-thaw sperm motility was found in sperm samples with concentration of 0.2 and 1 x 10(9) spz mL(-1). The sperm concentration of 3 x 10(9) spz mL(-1), which is higher than the native sperm concentration in sterlet, was found to be appropriate for use in cryopreservation procedures since the sperm fertilizing ability remained at a high level even though a significant decline in percentage of sperm motility was observed. These findings supported the conclusion that the procedure of artificially increasing the sperm concentration before freezing could be useful for the reduction of the volume kept in cryostorage and to decrease the sample volume required for artificial fertilization.

Název v anglickém jazyce

Optimization of sterlet sperm concentration for cryopreservation

Popis výsledku anglicky

One of the critical points in low temperature storage is the expense of storage capacities. Moreover, sperm concentration in a sample is not only a parameter for effective use of a cryobanks but also a parameter affecting cryopreservation outcomes. Sturgeon sperm in comparison to other species is significantly less concentrated due to the specificity of sperm maturation process, during which sperm is mixed with urine. In this study, we evaluated whether the artificial modification of the sterlet spermatozoa concentration before freezing can be useful during the routine application of sturgeon sperm cryopreservation. The sperm was first concentrated by centrifugation of native sperm samples. Then, the excess of seminal fluid was collected. The concentrated sperm samples were further diluted with the same seminal fluid to obtain different sperm concentrations in suspension, and they were subjected to freeze-thawing. The percentage of postthaw sperm motility depended on the sperm concentration in the samples. The highest post-thaw sperm motility was found in sperm samples with concentration of 0.2 and 1 x 10(9) spz mL(-1). The sperm concentration of 3 x 10(9) spz mL(-1), which is higher than the native sperm concentration in sterlet, was found to be appropriate for use in cryopreservation procedures since the sperm fertilizing ability remained at a high level even though a significant decline in percentage of sperm motility was observed. These findings supported the conclusion that the procedure of artificially increasing the sperm concentration before freezing could be useful for the reduction of the volume kept in cryostorage and to decrease the sample volume required for artificial fertilization.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

1873-5622

Svazek periodika

540

Číslo periodika v rámci svazku

neuveden

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

—

Kód UT WoS článku

000649693800001

EID výsledku v databázi Scopus

2-s2.0-85103737148