Practical use of extender and activation solutions for short-term storage of common carp (Cyprinus carpio) milt in a hatchery

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F22%3A43904535" target="_blank" >RIV/60076658:12520/22:43904535 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.aqrep.2022.101160" target="_blank" >https://doi.org/10.1016/j.aqrep.2022.101160</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aqrep.2022.101160" target="_blank" >10.1016/j.aqrep.2022.101160</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Practical use of extender and activation solutions for short-term storage of common carp (Cyprinus carpio) milt in a hatchery

Popis výsledku v původním jazyce

The purpose of this study was to investigate the effect of an activation solution (AS) (hatchery water (pH 7.9 and 4 mOsm/kg), Perchec solution (5 mM KCl, 45 mM NaCl, 30 mM Tris-HCl; pH 8.0 and 130 mOsm/kg) and Cheng solution (40 mM NaCl, 20 mM Tris; pH 8.0 and 100 mOsm/kg) on aging sperm of common carp (Cyprinus carpio) after storage in a carp artificial seminal plasma (carp ASP: 2 mM CaCl2, 1 mM MgSO4, 40 mM KCl, 110 mM NaCl, 20 mM Tris; pH 7.5 and 310 mOsm/kg) for 7 days without antibiotics at 4 degrees C. Fresh milt and undiluted aging milt were used as the control groups. Milt (5 ml) was collected and diluted 1:1 in a carp ASP in 50 ml containers and stored at 4 degrees C under aerobic conditions. The highest sperm motility was found 45% and 36% at 15 s and 45 s post sperm activation (PSA) in hatchery water. Additionally, the curvilinear velocity (VCL, mu m/s) and straight-line velocity (VSL, mu m/s) of sperm were the highest in hatchery water and the solution with 5 mM KCl, 45 mM NaCl, 30 mM Tris and pH 8.0, which were above 122 mu m/s and 87 mu m/s at 15 PSA. Sperm concentration in aging sperm of common carp is known to decrease rapidly, so prior to fertilization, sperm concentration was determined and accurately set to 200,000 sperm per egg. The fertilization of eggs and hatching rate after 7 days of sperm storage in carp ASP were 85-87% and 78-81% in both ASs of hatchery water and Perchec solution. Less than 1% sperm motility, fertilization and hatching rate were observed in undiluted sperm (control). In conclusion, the fertilization ability of aging common carp sperm in carp ASP within different ASs was confirmed for practical use in aquaculture.

Název v anglickém jazyce

Practical use of extender and activation solutions for short-term storage of common carp (Cyprinus carpio) milt in a hatchery

Popis výsledku anglicky

The purpose of this study was to investigate the effect of an activation solution (AS) (hatchery water (pH 7.9 and 4 mOsm/kg), Perchec solution (5 mM KCl, 45 mM NaCl, 30 mM Tris-HCl; pH 8.0 and 130 mOsm/kg) and Cheng solution (40 mM NaCl, 20 mM Tris; pH 8.0 and 100 mOsm/kg) on aging sperm of common carp (Cyprinus carpio) after storage in a carp artificial seminal plasma (carp ASP: 2 mM CaCl2, 1 mM MgSO4, 40 mM KCl, 110 mM NaCl, 20 mM Tris; pH 7.5 and 310 mOsm/kg) for 7 days without antibiotics at 4 degrees C. Fresh milt and undiluted aging milt were used as the control groups. Milt (5 ml) was collected and diluted 1:1 in a carp ASP in 50 ml containers and stored at 4 degrees C under aerobic conditions. The highest sperm motility was found 45% and 36% at 15 s and 45 s post sperm activation (PSA) in hatchery water. Additionally, the curvilinear velocity (VCL, mu m/s) and straight-line velocity (VSL, mu m/s) of sperm were the highest in hatchery water and the solution with 5 mM KCl, 45 mM NaCl, 30 mM Tris and pH 8.0, which were above 122 mu m/s and 87 mu m/s at 15 PSA. Sperm concentration in aging sperm of common carp is known to decrease rapidly, so prior to fertilization, sperm concentration was determined and accurately set to 200,000 sperm per egg. The fertilization of eggs and hatching rate after 7 days of sperm storage in carp ASP were 85-87% and 78-81% in both ASs of hatchery water and Perchec solution. Less than 1% sperm motility, fertilization and hatching rate were observed in undiluted sperm (control). In conclusion, the fertilization ability of aging common carp sperm in carp ASP within different ASs was confirmed for practical use in aquaculture.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture Reports

ISSN

2352-5134

e-ISSN

—

Svazek periodika

24

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

nestrankovano

Kód UT WoS článku

000799948200004

EID výsledku v databázi Scopus

2-s2.0-85129935516