Improvement of common carp artificial reproduction using enzyme for elimination of egg stickiness

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12610%2F03%3A00004991" target="_blank" >RIV/60076658:12610/03:00004991 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:_____/03:00004991

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Improvement of common carp artificial reproduction using enzyme for elimination of egg stickiness

Popis výsledku v původním jazyce

This study summarizes optimization of techniques for common carp artificial propagation including improvements of activation solution (AS), the process of insemination, and elimination of egg stickiness. The optimum gamete ratio for good fertilization and hatching rate ranged from 8490 to 23 672 spermatozoa per egg, when dechlorinated tap water was used. Optimal ratio between eggs (weight in g) and AS (in ml) was defined as 1: 1 to 1:2. Different concentrations of AS such as NaCl from 0 to 34 mM (0 68 mOsmol kg(-1).) did not change fertilization and hatching rates. An AS adopted for carp spermatozoa (45 mM NaCl, 5 mM KCl, 30 mM Tris-HCl, pH 8) was compared with other saline AS; only the 51 mM (102 mOsmol kg(-1)) NaCl solution decreased fertilization and hatching rate. The AS containing 20 mM Tris-HCl at pH 9 increased fertilization and hatching rates compared to dechlorinated tap water of pH 7 or to AS of pH 6 and 7. Adhesiveness from the eggs was successfully removed by incubation in

Název v anglickém jazyce

Improvement of common carp artificial reproduction using enzyme for elimination of egg stickiness

Popis výsledku anglicky

This study summarizes optimization of techniques for common carp artificial propagation including improvements of activation solution (AS), the process of insemination, and elimination of egg stickiness. The optimum gamete ratio for good fertilization and hatching rate ranged from 8490 to 23 672 spermatozoa per egg, when dechlorinated tap water was used. Optimal ratio between eggs (weight in g) and AS (in ml) was defined as 1: 1 to 1:2. Different concentrations of AS such as NaCl from 0 to 34 mM (0 68 mOsmol kg(-1).) did not change fertilization and hatching rates. An AS adopted for carp spermatozoa (45 mM NaCl, 5 mM KCl, 30 mM Tris-HCl, pH 8) was compared with other saline AS; only the 51 mM (102 mOsmol kg(-1)) NaCl solution decreased fertilization and hatching rate. The AS containing 20 mM Tris-HCl at pH 9 increased fertilization and hatching rates compared to dechlorinated tap water of pH 7 or to AS of pH 6 and 7. Adhesiveness from the eggs was successfully removed by incubation in

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GL - Rybářství

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquatic Living Resources

ISSN

0990-7440

e-ISSN

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Svazek periodika

16

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

6

Strana od-do

450-456

Kód UT WoS článku

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EID výsledku v databázi Scopus

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