Better intestine health in fast-growing bullfrogs (Lithobates catesbeianus) fed a soybean meal-based diet than slow-growing bullfrogs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F22%3A43904569" target="_blank" >RIV/60076658:12520/22:43904569 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1111/are.15978" target="_blank" >https://doi.org/10.1111/are.15978</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/are.15978" target="_blank" >10.1111/are.15978</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Better intestine health in fast-growing bullfrogs (Lithobates catesbeianus) fed a soybean meal-based diet than slow-growing bullfrogs

Popis výsledku v původním jazyce

This study examined the differences in intestinal health between fast- and slow-growing bullfrogs (Lithobates catesbeianus) fed a soybean meal (SM)-based diet. A total of 108 froglets (26.14 +/- 0.21 g) were randomly distributed into three tanks at a density of 36 froglets per tank and fed a SM-based diet for 8 weeks. At the end of the feeding period, four heaviest and four lightest bullfrogs from each tank were captured and considered as fast-growing (FG) and slow-growing (SG) groups. The FG group had significantly higher weight gain than the SG group (p &lt; 0.05). A basic intestinal tissue structure integrity with higher values of jejunal diameter, villus numbers, villus thickness and muscular thickness was observed in the FG group compared with the SG group (p &lt; 0.05). Moreover, lower activity of serum diamine oxidase was detected in FG group than the SG group, which indicated better gut barrier function of froglets in FG group (p &lt; 0.05). However, no significant differences were found in the mRNA expression level of tight junction proteins including occludin and zo-1 in the jejunum, and serum D-lactate concentration between the two groups (p &gt; 0.05). Significantly lower pH values in corpora ventriculi and ileum and higher jejunal protease and amylase activities were found in the FG group than SG group (p &lt; 0.05). Relatively higher expression of anti-inflammatory genes and lower expression of pro-inflammatory genes were found in the FG group; however, the differences were not significant. The abundances of potential probiotics including Akkermansia, Lactobacillus and Prevotellaceae_UCG_001 were higher in the intestinal microflora of FG group compared with the SG group. In conclusion, these findings suggested that higher growth of FG bullfrogs fed the SM-based diet could be attributed to their better intestinal morphological structure and barrier function compared with the SG bullfrogs.

Název v anglickém jazyce

Better intestine health in fast-growing bullfrogs (Lithobates catesbeianus) fed a soybean meal-based diet than slow-growing bullfrogs

Popis výsledku anglicky

This study examined the differences in intestinal health between fast- and slow-growing bullfrogs (Lithobates catesbeianus) fed a soybean meal (SM)-based diet. A total of 108 froglets (26.14 +/- 0.21 g) were randomly distributed into three tanks at a density of 36 froglets per tank and fed a SM-based diet for 8 weeks. At the end of the feeding period, four heaviest and four lightest bullfrogs from each tank were captured and considered as fast-growing (FG) and slow-growing (SG) groups. The FG group had significantly higher weight gain than the SG group (p &lt; 0.05). A basic intestinal tissue structure integrity with higher values of jejunal diameter, villus numbers, villus thickness and muscular thickness was observed in the FG group compared with the SG group (p &lt; 0.05). Moreover, lower activity of serum diamine oxidase was detected in FG group than the SG group, which indicated better gut barrier function of froglets in FG group (p &lt; 0.05). However, no significant differences were found in the mRNA expression level of tight junction proteins including occludin and zo-1 in the jejunum, and serum D-lactate concentration between the two groups (p &gt; 0.05). Significantly lower pH values in corpora ventriculi and ileum and higher jejunal protease and amylase activities were found in the FG group than SG group (p &lt; 0.05). Relatively higher expression of anti-inflammatory genes and lower expression of pro-inflammatory genes were found in the FG group; however, the differences were not significant. The abundances of potential probiotics including Akkermansia, Lactobacillus and Prevotellaceae_UCG_001 were higher in the intestinal microflora of FG group compared with the SG group. In conclusion, these findings suggested that higher growth of FG bullfrogs fed the SM-based diet could be attributed to their better intestinal morphological structure and barrier function compared with the SG bullfrogs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture Research

ISSN

1355-557X

e-ISSN

1365-2109

Svazek periodika

53

Číslo periodika v rámci svazku

13

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

4847-4857

Kód UT WoS článku

000822461300001

EID výsledku v databázi Scopus

2-s2.0-85133592073