Trehalose alleviated hepatic cholesterol accumulation via inhibiting transformation from glucose-derived acyl-CoA to cholesterol synthesis in Nile tilapia

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F22%3A43904875" target="_blank" >RIV/60076658:12520/22:43904875 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.aquaculture.2022.738600" target="_blank" >https://doi.org/10.1016/j.aquaculture.2022.738600</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2022.738600" target="_blank" >10.1016/j.aquaculture.2022.738600</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Trehalose alleviated hepatic cholesterol accumulation via inhibiting transformation from glucose-derived acyl-CoA to cholesterol synthesis in Nile tilapia

Popis výsledku v původním jazyce

Trehalose has been identified as an autophagy enhancer with atheroprotective properties in mammals that reduces cholesterol accumulation in the body. However, such effects have been rarely explored in aquatic animals. This study examined the effects of trehalose supplementation in a high-cholesterol diet on liver cholesterol accumulation and the related mechanisms in Nile tilapia (Oreochromis niloticus). The fish were fed high cholesterol (1.6%) diets supplemented with different levels (0, 1.5, 3 and 4.5%) of trehalose for eight weeks. The results showed that inclusion of 3% trehalose in high-cholesterol diet led to the significant reduction of viscerosomatic index (VSI), hepatosomatic index (HSI) and total and free cholesterol (TC and FC) accumulation in the serum and liver of fish. Moreover, 3% trehalose supplementation decreased serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, indicating the alleviation of high-cholesterol diet-induced liver impairment. Surprisingly, such beneficial effects did not correlate with autophagy activity since dietary inclusion of 3% trehalose inhibited the expression of autophagy-related genes and proteins (atg5/12, autophagy related gene 5/12; Lc3, microtubule-associated protein 1 light chain 3 and Beclin protein). Also, the same group inhibited the expression of genes related to hepatic cholesterol synthesis, glycogen metabolism, glycolysis, and glucose oxidative phosphorylation. These results indicated that trehalose treatment reduced the capability of glucose catabolism in the liver of high-cholesterol diet-fed fish. In the primary hepatocytes of Nile tilapia, which were cultured in a medium containing high cholesterol concentration, the [1-C-14]-glucose tracking test showed that trehalose treatment resulted in decreased cellular retention of C-14-glucose and unaltered release of (CO2)-C-14. Likewise, the trehalose treatment inhibited the expression of genes associated with glucose uptake and catabolism, cholesterol synthesis. Also, the inhibited autophagy activities were found in the primary hepatocytes treated with trehalose. Overall, trehalose treatment alleviated high-cholesterol diet-induced hepatic and serum cholesterol accumulation in Nile tilapia, but such effect was not associated with the autophagy activities. Alternatively, our results suggested that trehalose exerted a cholesterol-lowering effect via inhibiting the transformation from glucose-mediated acyl-CoA to cholesterol synthesis in the liver of fish. This is the first study revealing the cholesterol-lowering function of trehalose in fish and could contribute to developing new strategies for maintaining liver health in fish.

Název v anglickém jazyce

Trehalose alleviated hepatic cholesterol accumulation via inhibiting transformation from glucose-derived acyl-CoA to cholesterol synthesis in Nile tilapia

Popis výsledku anglicky

Trehalose has been identified as an autophagy enhancer with atheroprotective properties in mammals that reduces cholesterol accumulation in the body. However, such effects have been rarely explored in aquatic animals. This study examined the effects of trehalose supplementation in a high-cholesterol diet on liver cholesterol accumulation and the related mechanisms in Nile tilapia (Oreochromis niloticus). The fish were fed high cholesterol (1.6%) diets supplemented with different levels (0, 1.5, 3 and 4.5%) of trehalose for eight weeks. The results showed that inclusion of 3% trehalose in high-cholesterol diet led to the significant reduction of viscerosomatic index (VSI), hepatosomatic index (HSI) and total and free cholesterol (TC and FC) accumulation in the serum and liver of fish. Moreover, 3% trehalose supplementation decreased serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, indicating the alleviation of high-cholesterol diet-induced liver impairment. Surprisingly, such beneficial effects did not correlate with autophagy activity since dietary inclusion of 3% trehalose inhibited the expression of autophagy-related genes and proteins (atg5/12, autophagy related gene 5/12; Lc3, microtubule-associated protein 1 light chain 3 and Beclin protein). Also, the same group inhibited the expression of genes related to hepatic cholesterol synthesis, glycogen metabolism, glycolysis, and glucose oxidative phosphorylation. These results indicated that trehalose treatment reduced the capability of glucose catabolism in the liver of high-cholesterol diet-fed fish. In the primary hepatocytes of Nile tilapia, which were cultured in a medium containing high cholesterol concentration, the [1-C-14]-glucose tracking test showed that trehalose treatment resulted in decreased cellular retention of C-14-glucose and unaltered release of (CO2)-C-14. Likewise, the trehalose treatment inhibited the expression of genes associated with glucose uptake and catabolism, cholesterol synthesis. Also, the inhibited autophagy activities were found in the primary hepatocytes treated with trehalose. Overall, trehalose treatment alleviated high-cholesterol diet-induced hepatic and serum cholesterol accumulation in Nile tilapia, but such effect was not associated with the autophagy activities. Alternatively, our results suggested that trehalose exerted a cholesterol-lowering effect via inhibiting the transformation from glucose-mediated acyl-CoA to cholesterol synthesis in the liver of fish. This is the first study revealing the cholesterol-lowering function of trehalose in fish and could contribute to developing new strategies for maintaining liver health in fish.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

1873-5622

Svazek periodika

560

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

nestrankovano

Kód UT WoS článku

000880717300001

EID výsledku v databázi Scopus

2-s2.0-85133741738