Identification of hot spots and co-occurrence patterns of activities on thyroid hormone receptor and transthyretin binding in passive samplers from Czech surface waters

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F24%3A43908113" target="_blank" >RIV/60076658:12520/24:43908113 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00020699:_____/24:N0000025

Výsledek na webu

<a href="https://doi.org/10.1016/j.envres.2024.118891" target="_blank" >https://doi.org/10.1016/j.envres.2024.118891</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.envres.2024.118891" target="_blank" >10.1016/j.envres.2024.118891</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of hot spots and co-occurrence patterns of activities on thyroid hormone receptor and transthyretin binding in passive samplers from Czech surface waters

Popis výsledku v původním jazyce

One of the less studied in vitro biological activities in the aquatic environment are thyroid hormone receptor beta (TR beta)-mediated agonistic and antagonistic activities and transthyretin (TTR) binding activity. They were measured mostly using active sampling methods, but rarely found. It is unclear if these activities co-occur, and the drivers of the (anti-)TR beta activity are mostly unknown. Therefore, the main aim of the study was to determine (anti-)TR beta activities as well as transthyretin (TTR) binding activity in passive samplers from Czech surface waters in combination with the search for the effect drivers based on liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis by applying suspect screening. Passive samplers (polar organic chemical integrative samplers, POCIS) were deployed at twenty-one sites (all ends of watersheds and other important sites in Elbe River) in the Czech rivers. The (anti-)TR beta and TTR binding activity were measured using (anti-)TR beta CALUX and TTR-TR beta-CALUX bioassays. Anti-TR beta activity was found at eight sites, and TTR binding activity cooccurred there at six of these sites. The co-occurrence of TR beta-mediated antagonistic activity and TTR binding indicate that they may have common effect drivers. No sample exhibited TR beta agonistic activity. The extract from the site B &amp; iacute;lina River, the most burdened with anti-TR beta activity, was further successfully fractionated, and this activity was revealed in the fraction, where mid-polar compounds prevailed. However, the suspect LC-HRMS analysis did not reveal the chemical effect drivers. Our results showed that anti-TR beta activity can be found in surface waters by employing passive sampling and frequently co-occurs with TTR binding activity. Overall, the fractionation procedure and non-target data acquisition used in this study can serve as a basis for searching the effect drivers in future research.

Název v anglickém jazyce

Identification of hot spots and co-occurrence patterns of activities on thyroid hormone receptor and transthyretin binding in passive samplers from Czech surface waters

Popis výsledku anglicky

One of the less studied in vitro biological activities in the aquatic environment are thyroid hormone receptor beta (TR beta)-mediated agonistic and antagonistic activities and transthyretin (TTR) binding activity. They were measured mostly using active sampling methods, but rarely found. It is unclear if these activities co-occur, and the drivers of the (anti-)TR beta activity are mostly unknown. Therefore, the main aim of the study was to determine (anti-)TR beta activities as well as transthyretin (TTR) binding activity in passive samplers from Czech surface waters in combination with the search for the effect drivers based on liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis by applying suspect screening. Passive samplers (polar organic chemical integrative samplers, POCIS) were deployed at twenty-one sites (all ends of watersheds and other important sites in Elbe River) in the Czech rivers. The (anti-)TR beta and TTR binding activity were measured using (anti-)TR beta CALUX and TTR-TR beta-CALUX bioassays. Anti-TR beta activity was found at eight sites, and TTR binding activity cooccurred there at six of these sites. The co-occurrence of TR beta-mediated antagonistic activity and TTR binding indicate that they may have common effect drivers. No sample exhibited TR beta agonistic activity. The extract from the site B &amp; iacute;lina River, the most burdened with anti-TR beta activity, was further successfully fractionated, and this activity was revealed in the fraction, where mid-polar compounds prevailed. However, the suspect LC-HRMS analysis did not reveal the chemical effect drivers. Our results showed that anti-TR beta activity can be found in surface waters by employing passive sampling and frequently co-occurs with TTR binding activity. Overall, the fractionation procedure and non-target data acquisition used in this study can serve as a basis for searching the effect drivers in future research.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10511 - Environmental sciences (social aspects to be 5.7)

Projekt

<a href="/cs/project/GX20-04676X" target="_blank" >GX20-04676X: Holistická charakterizace expozice a potenciálních účinků komplexních směsí chemických látek ve vodním prostředí</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Research

ISSN

0013-9351

e-ISSN

1096-0953

Svazek periodika

252

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

—

Kód UT WoS článku

001228640100001

EID výsledku v databázi Scopus

2-s2.0-85189929263