Effects of α-lipoic acid supplementation in a high-fat diet on growth, lipid metabolism and mitochondrial function in spotted seabass (Lateolabrax maculatus)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F24%3A43908329" target="_blank" >RIV/60076658:12520/24:43908329 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.aqrep.2024.102408" target="_blank" >https://doi.org/10.1016/j.aqrep.2024.102408</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aqrep.2024.102408" target="_blank" >10.1016/j.aqrep.2024.102408</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of α-lipoic acid supplementation in a high-fat diet on growth, lipid metabolism and mitochondrial function in spotted seabass (Lateolabrax maculatus)

Popis výsledku v původním jazyce

The aim of this study was to explore the impacts of incorporating a-lipoic acid (LA) in a high-fat diet (HFD) on growth, lipid metabolism and mitochondrial function in Lateolabrax maculatus. A normal-fat diet (NFD, 10 % lipid), a high-fat diet (HFD, 16 % lipid) and three LA-added HFD (200, 400, or 600 mg kg(-1) LA) were prepared and fed to five group of L. maculatus for 57 days. Supplementation of 400 mg kg(-1) LA to the HFD improved growth performance and feed utilization. LA treatment reduced the lipids concentration in serum and liver. Moreover, application of 400 mg kg(-1) LA in HFD notably decreased the activity of transaminases in serum. Liver oil red O staining results revealed the larger area of red lipid droplets in HFD group compared to the NFD group. Also, abdominal fat HE staining results showed larger adipocytes in the HFD group. Interestingly, LA application led to a reduction in the area of red lipid droplets and a decrease in the size of adipocytes. Furthermore, LA addition alleviated the HFD-induced oxidative stress as identified by enhanced mitochondrial citrate synthase, isocitrate dehydrogenase, alpha-ketoglutarate dehydrogenase, and succinate dehydrogenase activities. Meanwhile, LA administration induced the activation of mitochondrial biogenesis, and up-regulated the expression of autophagy-related genes. In conclusion, LA incorporation into HFD for L. maculatus promoted growth performance, enhanced liver mitochondrial function, and mitigated excessive fat accumulation in the liver.

Název v anglickém jazyce

Effects of α-lipoic acid supplementation in a high-fat diet on growth, lipid metabolism and mitochondrial function in spotted seabass (Lateolabrax maculatus)

Popis výsledku anglicky

The aim of this study was to explore the impacts of incorporating a-lipoic acid (LA) in a high-fat diet (HFD) on growth, lipid metabolism and mitochondrial function in Lateolabrax maculatus. A normal-fat diet (NFD, 10 % lipid), a high-fat diet (HFD, 16 % lipid) and three LA-added HFD (200, 400, or 600 mg kg(-1) LA) were prepared and fed to five group of L. maculatus for 57 days. Supplementation of 400 mg kg(-1) LA to the HFD improved growth performance and feed utilization. LA treatment reduced the lipids concentration in serum and liver. Moreover, application of 400 mg kg(-1) LA in HFD notably decreased the activity of transaminases in serum. Liver oil red O staining results revealed the larger area of red lipid droplets in HFD group compared to the NFD group. Also, abdominal fat HE staining results showed larger adipocytes in the HFD group. Interestingly, LA application led to a reduction in the area of red lipid droplets and a decrease in the size of adipocytes. Furthermore, LA addition alleviated the HFD-induced oxidative stress as identified by enhanced mitochondrial citrate synthase, isocitrate dehydrogenase, alpha-ketoglutarate dehydrogenase, and succinate dehydrogenase activities. Meanwhile, LA administration induced the activation of mitochondrial biogenesis, and up-regulated the expression of autophagy-related genes. In conclusion, LA incorporation into HFD for L. maculatus promoted growth performance, enhanced liver mitochondrial function, and mitigated excessive fat accumulation in the liver.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10617 - Marine biology, freshwater biology, limnology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture Reports

ISSN

2352-5134

e-ISSN

—

Svazek periodika

39

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

—

Kód UT WoS článku

001335585100001

EID výsledku v databázi Scopus

2-s2.0-85206212510