Cryopreservation of common carp ( Cyprinus carpio L.) sperm: Insights into low-ionic cryoprotective medium mechanism and efficacy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F25%3A43909711" target="_blank" >RIV/60076658:12520/25:43909711 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.aquaculture.2024.741807" target="_blank" >https://doi.org/10.1016/j.aquaculture.2024.741807</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2024.741807" target="_blank" >10.1016/j.aquaculture.2024.741807</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cryopreservation of common carp ( Cyprinus carpio L.) sperm: Insights into low-ionic cryoprotective medium mechanism and efficacy

Popis výsledku v původním jazyce

The report explaining the processes and conditions common carp spermatozoa required for successful cryopreservation using a low-ionic cryoprotective medium was represented. The study aimed at the cryopreservation outcomes of common carp sperm using hypertonic and hypotonic cryoprotective media, focusing on spermatozoon volume changes during the equilibration period and offspring ploidy levels. The effect of two types of cryoprotective media high-ionic (200 mM glucose, 40 mM KCl, 30 mM TRIS, 10 % methanol) and low-ionic (60 mM NaCl, 2.9 mM sucrose, 11 % methanol, 2.5 % ethylene glycol), two volumes of samples and two different cryopreservation methods (0.5 ml straws on a polystyrene raft in a Styrofoam box with rapid cooling rates and 4.5 ml cryotubes in the programmable freezer with slow cooling rates) were assessed. The best post-thawed motility results of common carp spermatozoa after hypertonic shrinking in straws with high-ionic cryoprotective medium, a dilution ratio of 1:9 (44.91 +/- 9.98 %), and hypotonic swelling in cryotubes with low-ionic cryoprotective medium, a dilution ratio of 1:1 (47,33 +/- 7.85 %) were obtained. The key to successful sperm cryopreservation is matching a cryoprotective medium and cooling rates to maintain osmotic balance and protect cells against mechanical damage from ice crystals. The slow cooling rate and the synergy effect of methanol and ethylene glycol make it possible to apply a low-ionic cryoprotective medium in a big sample volume for cryopreservation of common carp sperm. No significant differences in malformations or ploidy levels were observed between larvae from fresh and cryopreserved sperm. These findings underscore the potential and benefits of applying a low-ionic cryopreservation medium for common carp artificial reproduction in aquaculture.

Název v anglickém jazyce

Cryopreservation of common carp ( Cyprinus carpio L.) sperm: Insights into low-ionic cryoprotective medium mechanism and efficacy

Popis výsledku anglicky

The report explaining the processes and conditions common carp spermatozoa required for successful cryopreservation using a low-ionic cryoprotective medium was represented. The study aimed at the cryopreservation outcomes of common carp sperm using hypertonic and hypotonic cryoprotective media, focusing on spermatozoon volume changes during the equilibration period and offspring ploidy levels. The effect of two types of cryoprotective media high-ionic (200 mM glucose, 40 mM KCl, 30 mM TRIS, 10 % methanol) and low-ionic (60 mM NaCl, 2.9 mM sucrose, 11 % methanol, 2.5 % ethylene glycol), two volumes of samples and two different cryopreservation methods (0.5 ml straws on a polystyrene raft in a Styrofoam box with rapid cooling rates and 4.5 ml cryotubes in the programmable freezer with slow cooling rates) were assessed. The best post-thawed motility results of common carp spermatozoa after hypertonic shrinking in straws with high-ionic cryoprotective medium, a dilution ratio of 1:9 (44.91 +/- 9.98 %), and hypotonic swelling in cryotubes with low-ionic cryoprotective medium, a dilution ratio of 1:1 (47,33 +/- 7.85 %) were obtained. The key to successful sperm cryopreservation is matching a cryoprotective medium and cooling rates to maintain osmotic balance and protect cells against mechanical damage from ice crystals. The slow cooling rate and the synergy effect of methanol and ethylene glycol make it possible to apply a low-ionic cryoprotective medium in a big sample volume for cryopreservation of common carp sperm. No significant differences in malformations or ploidy levels were observed between larvae from fresh and cryopreserved sperm. These findings underscore the potential and benefits of applying a low-ionic cryopreservation medium for common carp artificial reproduction in aquaculture.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

1873-5622

Svazek periodika

596

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

nestránkováno

Kód UT WoS článku

001350198000001

EID výsledku v databázi Scopus

2-s2.0-85207776428