Enzyme treatment for elimination of egg stickiness in tench (Tinca tinca L.) European catfish (Silurus glanis L.) and common carp (Cyprinus carpio L.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12610%2F03%3A00004990" target="_blank" >RIV/60076658:12610/03:00004990 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Enzyme treatment for elimination of egg stickiness in tench (Tinca tinca L.) European catfish (Silurus glanis L.) and common carp (Cyprinus carpio L.)

Popis výsledku v původním jazyce

Enzyme treatment to eliminate egg stickiness in tench, European catfish and common carp was compared with standard methodology in an attempt to decrease time consuming under hatchery conditions. Eggs of tench and European catfish were exposed to an alcalase enzyme MERCK EC 3.4.21.14 solution 3 minutes after egg activationfor 2 minutes. In tench the highes hatching rate (87.1%) was found with 10,0 ml.l-1 enzyme treatment. The traditional desticking procedure in tench involving milk/clay treatment gave ahatching rate of 74.1% and required 1 hour. The best enzyme concentration in European catfish was 20 ml of enzyme per litre. The traditional desticking procedure in European catfish clay treatment gave a similar hatching rate but procedure required 30 minutes. The eggs of common carp were successfully prevented from stickiness with ALCALASE DX (PLN 04715) using two applications of enzyme from 8 to 20 minutes after fertilization at two level of enzyme concentration (2 ml . l-1 and 20 ml.l

Název v anglickém jazyce

Enzyme treatment for elimination of egg stickiness in tench (Tinca tinca L.) European catfish (Silurus glanis L.) and common carp (Cyprinus carpio L.)

Popis výsledku anglicky

Enzyme treatment to eliminate egg stickiness in tench, European catfish and common carp was compared with standard methodology in an attempt to decrease time consuming under hatchery conditions. Eggs of tench and European catfish were exposed to an alcalase enzyme MERCK EC 3.4.21.14 solution 3 minutes after egg activationfor 2 minutes. In tench the highes hatching rate (87.1%) was found with 10,0 ml.l-1 enzyme treatment. The traditional desticking procedure in tench involving milk/clay treatment gave ahatching rate of 74.1% and required 1 hour. The best enzyme concentration in European catfish was 20 ml of enzyme per litre. The traditional desticking procedure in European catfish clay treatment gave a similar hatching rate but procedure required 30 minutes. The eggs of common carp were successfully prevented from stickiness with ALCALASE DX (PLN 04715) using two applications of enzyme from 8 to 20 minutes after fertilization at two level of enzyme concentration (2 ml . l-1 and 20 ml.l

Druh

D - Stať ve sborníku

CEP obor

GL - Rybářství

OECD FORD obor

—

Projekt

<a href="/cs/project/GA524%2F03%2F0178" target="_blank" >GA524/03/0178: Detailní studium spermií u modelových druhů chrupavčitých (Chondrostei) a kostnatých (Teleostei) ryb</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Book of abstracts. 7th Int. Symposium on Reproductive Physiology of Fish

ISBN

—

ISSN

—

e-ISSN

—

Počet stran výsledku

1

Strana od-do

130

Název nakladatele

National Research Institute of Aquaculture, Fisheries Research Agency

Místo vydání

Mie

Místo konání akce

Mie

Datum konání akce

18. 5. 2003

Typ akce podle státní příslušnosti

—

Kód UT WoS článku

—