Kompetice spermií během in vitro oplození u kapra obecného(Cyprinus carpio L.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12610%2F08%3A00008901" target="_blank" >RIV/60076658:12610/08:00008901 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12610/08:00008904

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cryopreservation of sperm of testicular neomales and stripped normal males of European perch (Perca fluviatilis L.)

Popis výsledku v původním jazyce

The aim of this in vitro fertilization study was to compare competitive success of five males using four different sperm/egg ratios. Sperm concentrations were evaluated for five males of common carp (M1-M5) to compose pool of sperm (heterosperm) with equal number of sperm of each of males. Four batches of eggs, each of 5g (800pcs/g), were fertilized using volume of heterosperm relevant to the ratios of 5000 (G1), 10 000 (G2), 20 000 (G3) and 100 000 (G4) spermatozoa per egg. The fertilized eggs were individually incubated (in four groups G1?G4). From 54 to 91 individuals were sampled for paternity analysis after incubation. Genotyping of four microsatellite loci (MFW1, MFW6, MFW7, MFW28) with an automated sequencing device was used to determine the paternity in four groups of progenies. The motility of spermatozoa was recorded and analysed. Fertilization (23.67 - 94.82 %) and hatching (23.67 - 92.22 %) control rates of individual males were counted. Sperm velocity and percentage of mot

Název v anglickém jazyce

Cryopreservation of sperm of testicular neomales and stripped normal males of European perch (Perca fluviatilis L.)

Popis výsledku anglicky

The aim of this in vitro fertilization study was to compare competitive success of five males using four different sperm/egg ratios. Sperm concentrations were evaluated for five males of common carp (M1-M5) to compose pool of sperm (heterosperm) with equal number of sperm of each of males. Four batches of eggs, each of 5g (800pcs/g), were fertilized using volume of heterosperm relevant to the ratios of 5000 (G1), 10 000 (G2), 20 000 (G3) and 100 000 (G4) spermatozoa per egg. The fertilized eggs were individually incubated (in four groups G1?G4). From 54 to 91 individuals were sampled for paternity analysis after incubation. Genotyping of four microsatellite loci (MFW1, MFW6, MFW7, MFW28) with an automated sequencing device was used to determine the paternity in four groups of progenies. The motility of spermatozoa was recorded and analysed. Fertilization (23.67 - 94.82 %) and hatching (23.67 - 92.22 %) control rates of individual males were counted. Sperm velocity and percentage of mot

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA524%2F06%2F0817" target="_blank" >GA524/06/0817: Ultrastruktura, energie a kompetice spermií: Komparativní studie s využitím dvou modelových druhů chrupavčitých a kostnatých ryb</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cybium

ISSN

0399-0974

e-ISSN

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Svazek periodika

32

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

4

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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