Cross-krystalizace jako nová optimalizační krystalizační metoda

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12640%2F07%3A00008317" target="_blank" >RIV/60076658:12640/07:00008317 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cross-crystallization as a new optimization tool of crystallization procedures

Popis výsledku v původním jazyce

The effect of several metal cations Cu2+, Cd2+, Co2+, Ba2+) was tested in attempts to improve crystallization procedure and verify a newly discovered cross-crystallization method with two selected proteins; di-heme cytochrome c4 from anaerobic purple sulphur bacterium Thiocapsa roseopersicina and sweet-tasting protein thaumatin from the African berry Thaumatococcus daniellii. Presence of Cu2+ ions promoted dramatic improvement in crystal morphology, internal packing and diffraction quality. This investigation qualitatively established the influence of cupric cations on the crystal growth by using the cross-crystallization procedure. It was foud that influence of Cu2+ ions produced evidently different outer morphology and internal packing of thaumatin crystals (hexagonal prism). Usually their shape is presented as a tetragonal bipyramids. In the case of cytochrome, the good diffractable crystals were obtained only by using cross-crystallization method with metal-ion salts. Newly grown c

Název v anglickém jazyce

Cross-crystallization as a new optimization tool of crystallization procedures

Popis výsledku anglicky

The effect of several metal cations Cu2+, Cd2+, Co2+, Ba2+) was tested in attempts to improve crystallization procedure and verify a newly discovered cross-crystallization method with two selected proteins; di-heme cytochrome c4 from anaerobic purple sulphur bacterium Thiocapsa roseopersicina and sweet-tasting protein thaumatin from the African berry Thaumatococcus daniellii. Presence of Cu2+ ions promoted dramatic improvement in crystal morphology, internal packing and diffraction quality. This investigation qualitatively established the influence of cupric cations on the crystal growth by using the cross-crystallization procedure. It was foud that influence of Cu2+ ions produced evidently different outer morphology and internal packing of thaumatin crystals (hexagonal prism). Usually their shape is presented as a tetragonal bipyramids. In the case of cytochrome, the good diffractable crystals were obtained only by using cross-crystallization method with metal-ion salts. Newly grown c

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/LC06010" target="_blank" >LC06010: Centrum biokatalýzy a biotransformací</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials Structure

ISSN

1211-5894

e-ISSN

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Svazek periodika

2007

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

3

Strana od-do

3-5

Kód UT WoS článku

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EID výsledku v databázi Scopus

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