Cryopreservation of sperm in common carp Cyprinus carpio: correlation of sperm motility and hatching of embryos

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A_____%2F02%3A00004204" target="_blank" >RIV/60076658:_____/02:00004204 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12610/02:00003886 RIV/60076658:12610/02:00005782

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cryopreservation of sperm in common carp Cyprinus carpio: correlation of sperm motility and hatching of embryos

Popis výsledku v původním jazyce

In this study, cryopreservation methods were elaborated for ex situ conservation of nine strains of Bohemian common carp. A sperm freezing procedure was developed that resulted in high motility after thawing as well as high values of velocity and percentage of sperm motility, fertilization and hatching yield. Common carp sperm were diluted in Kurokura medium, chilled to 4 oC and dimethylsulfoxide (Me2SO) was added. Cryotubes of sperm with media were then cooled from +4 oC to -9 oC at a rate of 4 oC.min-1, then from -9 oC to -80 oC at a rate of 11 oC.min-1, held for 6 min at -80 oC and finally transferred into liquid N2. The spermatozoa were thawed in a water bath at 35 oC for 110 s and checked for fertilization yield, hatching yield of embryos and larval malformations. Fresh and frozen/thawed sperm were evaluated for the percentage and for the velocity of motile sperm from video frames using image analysis (Linhart et al., 2000). The means with standard deviation (SD) were evaluated fr

Název v anglickém jazyce

Cryopreservation of sperm in common carp Cyprinus carpio: correlation of sperm motility and hatching of embryos

Popis výsledku anglicky

In this study, cryopreservation methods were elaborated for ex situ conservation of nine strains of Bohemian common carp. A sperm freezing procedure was developed that resulted in high motility after thawing as well as high values of velocity and percentage of sperm motility, fertilization and hatching yield. Common carp sperm were diluted in Kurokura medium, chilled to 4 oC and dimethylsulfoxide (Me2SO) was added. Cryotubes of sperm with media were then cooled from +4 oC to -9 oC at a rate of 4 oC.min-1, then from -9 oC to -80 oC at a rate of 11 oC.min-1, held for 6 min at -80 oC and finally transferred into liquid N2. The spermatozoa were thawed in a water bath at 35 oC for 110 s and checked for fertilization yield, hatching yield of embryos and larval malformations. Fresh and frozen/thawed sperm were evaluated for the percentage and for the velocity of motile sperm from video frames using image analysis (Linhart et al., 2000). The means with standard deviation (SD) were evaluated fr

Druh

D - Stať ve sborníku

CEP obor

GL - Rybářství

OECD FORD obor

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Projekt

<a href="/cs/project/GV523%2F97%2FS056" target="_blank" >GV523/97/S056: Populační diversita kapra obecného v České republice. Detailní genetická a reprodukční charakterizace populací</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

XXth Genetics Days (Proceedings of the Intenational Scientifics Conference

ISBN

80-7157-607-7

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

272-273

Název nakladatele

Mendelova zemědělská a lesnická univerzita

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

12. 9. 2002

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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