Test of modification of KUROKURA solution as imobilizing medium for spermatozoa of tench

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A_____%2F02%3A00004277" target="_blank" >RIV/60076658:_____/02:00004277 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12610/02:00005818

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Test of modification of KUROKURA solution as imobilizing medium for spermatozoa of tench

Popis výsledku v původním jazyce

When sampled, sperm of tench is more or less contaminated with urine what causes worsened spermatozoa motility and thus results in worsened fertilization rate. This phenomenon was described by Linhart et al. (1992, 2002). In order to maintain motility and fertilization capacity of spermatozoa, a technique was elaborated to collect sperm into immobilizing solution (Linhart et al., 1992). Based upon previous unpublished experiments, KUROKURA solution (Kurokura et al.,1984) and its modifications were tested in this study as immobilizing solutions for sampling and preservation of tench spermatozoa. Sperm was a pool obtained of 7 mature males of 800 g mean body weight, 24 h after hormonal stimulation with intramuscularly administered carp pituitary in doseof 2 mg per 1 kg body weight. Sperm was sampled by means of a syringe directly into immobilizing solution (IS), maintaining the ratio of IS : sperm as 2 : 1. Sperm was maintained in the syringes placed in thermostable boxes with ice at 0

Název v anglickém jazyce

Test of modification of KUROKURA solution as imobilizing medium for spermatozoa of tench

Popis výsledku anglicky

When sampled, sperm of tench is more or less contaminated with urine what causes worsened spermatozoa motility and thus results in worsened fertilization rate. This phenomenon was described by Linhart et al. (1992, 2002). In order to maintain motility and fertilization capacity of spermatozoa, a technique was elaborated to collect sperm into immobilizing solution (Linhart et al., 1992). Based upon previous unpublished experiments, KUROKURA solution (Kurokura et al.,1984) and its modifications were tested in this study as immobilizing solutions for sampling and preservation of tench spermatozoa. Sperm was a pool obtained of 7 mature males of 800 g mean body weight, 24 h after hormonal stimulation with intramuscularly administered carp pituitary in doseof 2 mg per 1 kg body weight. Sperm was sampled by means of a syringe directly into immobilizing solution (IS), maintaining the ratio of IS : sperm as 2 : 1. Sperm was maintained in the syringes placed in thermostable boxes with ice at 0

Druh

D - Stať ve sborníku

CEP obor

GL - Rybářství

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

XXth Genetics Days (Proceedings of the Intenational Scientifics Conference

ISBN

80-7157-607-7

ISSN

—

e-ISSN

—

Počet stran výsledku

3

Strana od-do

326-328

Název nakladatele

Mendelova zemědělská a lesnická univerzita

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

12. 9. 2002

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

—