Vliv interferonu gama a specifických polyklonálních protilátek na vývoj infekce Encephalitozoon cuniculi v myších peritoneálních makrofázích a v buněčné linii PMJ2-R

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F07%3A00089125" target="_blank" >RIV/60077344:_____/07:00089125 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/07:00008012

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effects of interferon gamma and specific polyclonal antibody on the infection of murine peritoneal macrophages and murine macrophage cell line PMJ2-R with Encephalitozoon cuniculi

Popis výsledku v původním jazyce

Activation of peritoneal macrophages by interferon gamma (IFN-gamma) resulted in the inhibition of Encephalitozoon cuniculi replication. E. cuniculi could replicate either in a non-activated cell line of murine macrophages PMJ2-R or in IFN-gamma-activated PMJ2-R cells. Moreover, activation with IFN-gamma led to faster replication of E. cuniculi in these cells. Opsonisation of E. cuniculi spores with anti-E. cuniculi polyclonal antibody did not affect E. cuniculi replication in both, non-activated and activated murine macrophages. Opsonisation of E. cuniculi spores caused the most effective replication of E. cuniculi in activated PMJ2-R cells. Results support the hypothesis that E. cuniculi uses phagocytosis for the infection of host cells. They also indicate that the mechanism by which spores of E. cuniculi are killed by macrophages is not dependent on nitric oxide and they reveal that PMJ2-R cells cannot substitute peritoneal murine macrophages in immunological studies on E. cuniculi.

Název v anglickém jazyce

Effects of interferon gamma and specific polyclonal antibody on the infection of murine peritoneal macrophages and murine macrophage cell line PMJ2-R with Encephalitozoon cuniculi

Popis výsledku anglicky

Activation of peritoneal macrophages by interferon gamma (IFN-gamma) resulted in the inhibition of Encephalitozoon cuniculi replication. E. cuniculi could replicate either in a non-activated cell line of murine macrophages PMJ2-R or in IFN-gamma-activated PMJ2-R cells. Moreover, activation with IFN-gamma led to faster replication of E. cuniculi in these cells. Opsonisation of E. cuniculi spores with anti-E. cuniculi polyclonal antibody did not affect E. cuniculi replication in both, non-activated and activated murine macrophages. Opsonisation of E. cuniculi spores caused the most effective replication of E. cuniculi in activated PMJ2-R cells. Results support the hypothesis that E. cuniculi uses phagocytosis for the infection of host cells. They also indicate that the mechanism by which spores of E. cuniculi are killed by macrophages is not dependent on nitric oxide and they reveal that PMJ2-R cells cannot substitute peritoneal murine macrophages in immunological studies on E. cuniculi.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

<a href="/cs/project/GP524%2F03%2FD167" target="_blank" >GP524/03/D167: Úloha IFN-gama v imunitní odpovědi při mikrosporidiové infekci</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Parasitologica

ISSN

0015-5683

e-ISSN

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Svazek periodika

54

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

172-176

Kód UT WoS článku

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EID výsledku v databázi Scopus

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