Zachovaná syntenie genů mezi chromosomem 15 u Bombyx mori a jedním chromosomem u Manduca sexta prokázaná pětibarevnou BAC-FISH

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F07%3A00090842" target="_blank" >RIV/60077344:_____/07:00090842 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/07:00008830

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Conserved synteny of genes between chromosome 15 of Bombyx mori and a chromosome of Manduca sexta shown by five-color BAC-FISH

Popis výsledku v původním jazyce

Conserved synteny of genes between particular chromosomes can be identified by comparatively mapping orthologous genes of the corresponding linkage groups with the help of BAC-FISH. This technique was established in Bombyx mori for two differently labeled BAC probes simultaneously hybridized to pachytene bivalents. In order to achieve higher-throughput comparative mapping using BAC-FISH in Lepidoptera, we developed a protocol for five-color BAC-FISH, which allowed us to map simultaneously six differentBAC probes to chromosome 15 in B. mori. We identified orthologs of six B. mori LG15 genes (RpP0, RpS8, RpL7A, eIF3, RpS23, and Hsc70) for the tobacco hornworm, Manduca sexta, and selected the ortholog-containing BAC clones from an M. sexta BAC library. All six M. sexta BAC clones hybridized to a single M. sexta bivalent in pachytene spermatocytes. Thus, we have confirmed the conserved synteny between the B. mori chromosome 15 and the corresponding M. sexta chromosome.

Název v anglickém jazyce

Conserved synteny of genes between chromosome 15 of Bombyx mori and a chromosome of Manduca sexta shown by five-color BAC-FISH

Popis výsledku anglicky

Conserved synteny of genes between particular chromosomes can be identified by comparatively mapping orthologous genes of the corresponding linkage groups with the help of BAC-FISH. This technique was established in Bombyx mori for two differently labeled BAC probes simultaneously hybridized to pachytene bivalents. In order to achieve higher-throughput comparative mapping using BAC-FISH in Lepidoptera, we developed a protocol for five-color BAC-FISH, which allowed us to map simultaneously six differentBAC probes to chromosome 15 in B. mori. We identified orthologs of six B. mori LG15 genes (RpP0, RpS8, RpL7A, eIF3, RpS23, and Hsc70) for the tobacco hornworm, Manduca sexta, and selected the ortholog-containing BAC clones from an M. sexta BAC library. All six M. sexta BAC clones hybridized to a single M. sexta bivalent in pachytene spermatocytes. Thus, we have confirmed the conserved synteny between the B. mori chromosome 15 and the corresponding M. sexta chromosome.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA206%2F06%2F1860" target="_blank" >GA206/06/1860: Molekulární evoluce pohlavních chromosomů motýlů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>O - Projekt operacniho programu

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Genome

ISSN

0831-2796

e-ISSN

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Svazek periodika

50

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

CA - Kanada

Počet stran výsledku

5

Strana od-do

1061-1065

Kód UT WoS článku

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EID výsledku v databázi Scopus

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