Extensive conserved synteny of genes between the karyotypes of Manduca sexta and Bombyx mori revealed by BAC-FISH mapping

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F09%3A00330525" target="_blank" >RIV/60077344:_____/09:00330525 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Extensive conserved synteny of genes between the karyotypes of Manduca sexta and Bombyx mori revealed by BAC-FISH mapping

Popis výsledku v původním jazyce

We used fluorescence in situ hybridization (FISH) with bacterial artificial chromosome (BAC) probes for physical mapping of genes and comparative genome analysis in Lepidoptera. We isolated BAC clones containing orthologs of single-copy genes of the silkworm, Bombyx mori, from a BAC library of the tobacco hornworm, Manduca sexta. We then constructed a BAC-FISH karyotype identifying all 28 chromosomes of M. sexta by mapping 124 loci using the corresponding BAC clones. BACprobes from M. sexta also generated clear signals on the corresponding chromosomes of a related species, Agrius convolvuli. Comparison of the M. sexta BAC physical map with the linkage map of B. mori pointed to conserved synteny including conserved gene order in most chromosomes. Only three inversions, three translocations, and two fission/fusion events were estimated to have occurred after the divergence of Bombycidae and Sphingidae. These results add to accumulating evidence for the stability of lepidopteran genomes.

Název v anglickém jazyce

Extensive conserved synteny of genes between the karyotypes of Manduca sexta and Bombyx mori revealed by BAC-FISH mapping

Popis výsledku anglicky

We used fluorescence in situ hybridization (FISH) with bacterial artificial chromosome (BAC) probes for physical mapping of genes and comparative genome analysis in Lepidoptera. We isolated BAC clones containing orthologs of single-copy genes of the silkworm, Bombyx mori, from a BAC library of the tobacco hornworm, Manduca sexta. We then constructed a BAC-FISH karyotype identifying all 28 chromosomes of M. sexta by mapping 124 loci using the corresponding BAC clones. BACprobes from M. sexta also generated clear signals on the corresponding chromosomes of a related species, Agrius convolvuli. Comparison of the M. sexta BAC physical map with the linkage map of B. mori pointed to conserved synteny including conserved gene order in most chromosomes. Only three inversions, three translocations, and two fission/fusion events were estimated to have occurred after the divergence of Bombycidae and Sphingidae. These results add to accumulating evidence for the stability of lepidopteran genomes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS ONE

ISSN

1932-6203

e-ISSN

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Svazek periodika

4

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000270847800007

EID výsledku v databázi Scopus

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