Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle in South Bohemia, the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F09%3A00334941" target="_blank" >RIV/60077344:_____/09:00334941 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/09:00010592 RIV/60076658:12220/09:00010836

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle in South Bohemia, the Czech Republic

Popis výsledku v původním jazyce

A total of 995 faecal samples from slaughtered dairy cattle was examined microscopically for the presence of Cryptosporidium spp. Nested PCR amplifying the partial SSU rRNA and GP60 genes of Cryptosporidium was performed on all positive and 200 randomlyselected samples and the products were digested by SspI, VspI and MboII restriction enzymes. The highest prevalence of infection (18.2%) was in the animals in age group of 12-18 months. The sequence analyses of PCR-positive specimens identified 41 samples as C. andersoni (4.1%), 2 samples as C. bovis (0.2%), and 1 sample as C. parvum (0.1%). Cryptosporidium bovis was detected only in a group of cattle older than 18 months and C. parvum in heifer which was older than 14 months. Infection intensity was generally low. Sequence analysis of the GP60 gene showed that detected C. parvum belonged to the IIaA16G1R1 subtype. This is the first published report about subtyping of the Cryptosporidium GP60 gene from cattle in the Czech Republic.

Název v anglickém jazyce

Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle in South Bohemia, the Czech Republic

Popis výsledku anglicky

A total of 995 faecal samples from slaughtered dairy cattle was examined microscopically for the presence of Cryptosporidium spp. Nested PCR amplifying the partial SSU rRNA and GP60 genes of Cryptosporidium was performed on all positive and 200 randomlyselected samples and the products were digested by SspI, VspI and MboII restriction enzymes. The highest prevalence of infection (18.2%) was in the animals in age group of 12-18 months. The sequence analyses of PCR-positive specimens identified 41 samples as C. andersoni (4.1%), 2 samples as C. bovis (0.2%), and 1 sample as C. parvum (0.1%). Cryptosporidium bovis was detected only in a group of cattle older than 18 months and C. parvum in heifer which was older than 14 months. Infection intensity was generally low. Sequence analysis of the GP60 gene showed that detected C. parvum belonged to the IIaA16G1R1 subtype. This is the first published report about subtyping of the Cryptosporidium GP60 gene from cattle in the Czech Republic.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/GP523%2F07%2FP117" target="_blank" >GP523/07/P117: Zoonotický potenciál kryptosporidií infikujících skot a prasata</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Parasitology

ISSN

0304-4017

e-ISSN

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Svazek periodika

165

Číslo periodika v rámci svazku

1/2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

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Kód UT WoS článku

000271160200021

EID výsledku v databázi Scopus

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