Molecular characterization of Cryptosporidium spp. in pre-weaned dairy calves in the Czech Republic: Absence of C. ryanae and management-associated distribution of C. andersoni, C. bovis and C. parvum subtypes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00359225" target="_blank" >RIV/60077344:_____/11:00359225 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12220/11:43882602 RIV/60076658:12510/11:43882602

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.vetpar.2010.11.048" target="_blank" >http://dx.doi.org/10.1016/j.vetpar.2010.11.048</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vetpar.2010.11.048" target="_blank" >10.1016/j.vetpar.2010.11.048</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular characterization of Cryptosporidium spp. in pre-weaned dairy calves in the Czech Republic: Absence of C. ryanae and management-associated distribution of C. andersoni, C. bovis and C. parvum subtypes

Popis výsledku v původním jazyce

A total of 750 faecal samples of dairy calves kept in various housing systems were screened for Cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method.DNAwas extracted from Cryptosporidium positive samples and from 150 randomly selected microscopically negative samples. Nested PCR was performed to amplify the partial SSU rRNA gene that was subsequently digested by SspI, VspI and MboII restriction enzymes. In addition, the C. parvum samples were subsequently analyzed at theGP60 gene to determine the distribution of zoonotic subtypes. Sequence analyses and RFLP identified C. parvum in 137, C. andersoni in 21 and C. bovis in 3 samples. Neither mixed infections nor C. ryanae was detected. IIaA15G2R1 and IIaA16G1R1 were detected as major subtypes. 3 of the 5 detected subtypes were previously associated with human cryptosporidiosis, and moreover, the IIaA15G1R1 subtype, previously reported in humans only, was detected in calves for the first time.

Název v anglickém jazyce

Molecular characterization of Cryptosporidium spp. in pre-weaned dairy calves in the Czech Republic: Absence of C. ryanae and management-associated distribution of C. andersoni, C. bovis and C. parvum subtypes

Popis výsledku anglicky

A total of 750 faecal samples of dairy calves kept in various housing systems were screened for Cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method.DNAwas extracted from Cryptosporidium positive samples and from 150 randomly selected microscopically negative samples. Nested PCR was performed to amplify the partial SSU rRNA gene that was subsequently digested by SspI, VspI and MboII restriction enzymes. In addition, the C. parvum samples were subsequently analyzed at theGP60 gene to determine the distribution of zoonotic subtypes. Sequence analyses and RFLP identified C. parvum in 137, C. andersoni in 21 and C. bovis in 3 samples. Neither mixed infections nor C. ryanae was detected. IIaA15G2R1 and IIaA16G1R1 were detected as major subtypes. 3 of the 5 detected subtypes were previously associated with human cryptosporidiosis, and moreover, the IIaA15G1R1 subtype, previously reported in humans only, was detected in calves for the first time.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Parasitology

ISSN

0304-4017

e-ISSN

—

Svazek periodika

177

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

378-382

Kód UT WoS článku

000290696800026

EID výsledku v databázi Scopus

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