Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F09%3A00334944" target="_blank" >RIV/60077344:_____/09:00334944 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/09:00010582

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein

Popis výsledku v původním jazyce

All mitochondrial tRNA genes are encoded in nucleus of T. brucei and thus all tRNAs have to be imported from the cytosol. We have investigated that silencing of the cysteine desulfurase, TbNfs, the key enzyme in tRNA thiolation (s2U) and Fe-S cluster formation in vivo, has no effect on tRNA partitioning. This is especially surprising in light of a recent report suggesting that in L. tropica the Rieske Fe-S protein is an essential component of the RNA import complex. We also show that down-regulation ofthe Rieske protein by RNA interference, similar to the TbNfs knockdowns, has no effect on import. The data presented here supports: s(2)U is not a negative determinant for tRNA import; the Rieske protein is not an essential component of the import machinery, and since the Rieske protein is essential for respiration and maintenance of inner mitochondrial membrane potential, neither process plays a critical role in tRNA import.

Název v anglickém jazyce

Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein

Popis výsledku anglicky

All mitochondrial tRNA genes are encoded in nucleus of T. brucei and thus all tRNAs have to be imported from the cytosol. We have investigated that silencing of the cysteine desulfurase, TbNfs, the key enzyme in tRNA thiolation (s2U) and Fe-S cluster formation in vivo, has no effect on tRNA partitioning. This is especially surprising in light of a recent report suggesting that in L. tropica the Rieske Fe-S protein is an essential component of the RNA import complex. We also show that down-regulation ofthe Rieske protein by RNA interference, similar to the TbNfs knockdowns, has no effect on import. The data presented here supports: s(2)U is not a negative determinant for tRNA import; the Rieske protein is not an essential component of the import machinery, and since the Rieske protein is essential for respiration and maintenance of inner mitochondrial membrane potential, neither process plays a critical role in tRNA import.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

R N A

ISSN

1355-8382

e-ISSN

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Svazek periodika

15

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000267142400017

EID výsledku v databázi Scopus

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