Glycobiochemistry of ticks, vectors of infectious diseases: carbohydrate-binding proteins and glycans

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F09%3A00336484" target="_blank" >RIV/60077344:_____/09:00336484 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Glycobiochemistry of ticks, vectors of infectious diseases: carbohydrate-binding proteins and glycans

Popis výsledku v původním jazyce

The lectin IxoA and enzyme chitinase 1 are potential factors of innate immunity in I. ricinus, especially active against bacterial and fungal infections. We succesfully knock-down the genes for both IxoA and Chix1 by RNA inhibition. Hemolymph of IxoA knock-down ticks totally lost its hemagglutination activity and the function of both genes is further studied by RNAi. Glycoproteins and sialic acid (SA) in salivary glands (SG) of the tick Ixodes ricinus: SG in ticks are of fundamental significance for thepathogen transmission to the host, amongst others. We mapped the glycoproteins of tick SG, potential receptors for the transmitted pathogens. We identified SA in glycoproteome of SG as well as a protein binding this carbohydrate. Based on the molecularfeatures of the protein, it is similar to mouse sialoadhesin Siglec-1.

Název v anglickém jazyce

Glycobiochemistry of ticks, vectors of infectious diseases: carbohydrate-binding proteins and glycans

Popis výsledku anglicky

The lectin IxoA and enzyme chitinase 1 are potential factors of innate immunity in I. ricinus, especially active against bacterial and fungal infections. We succesfully knock-down the genes for both IxoA and Chix1 by RNA inhibition. Hemolymph of IxoA knock-down ticks totally lost its hemagglutination activity and the function of both genes is further studied by RNAi. Glycoproteins and sialic acid (SA) in salivary glands (SG) of the tick Ixodes ricinus: SG in ticks are of fundamental significance for thepathogen transmission to the host, amongst others. We mapped the glycoproteins of tick SG, potential receptors for the transmitted pathogens. We identified SA in glycoproteome of SG as well as a protein binding this carbohydrate. Based on the molecularfeatures of the protein, it is similar to mouse sialoadhesin Siglec-1.

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/LC06009" target="_blank" >LC06009: Centrum molekulární ekologie vektorů a patogenů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů