Cloning and Molecular Analyses of Hop Transcription Factors

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cloning and Molecular Analyses of Hop Transcription Factors

Popis výsledku v původním jazyce

Expression of hop chs_H1 and other hop chalcon synthase (CHS)-like enzymes was assayed in developing pollen tissue. Both chs_H1 and valerophenone synthase (vps) are highly expressed in pollen suggesting the existence of common regulatory element(s). While vps is specific for female and male floral tissues, the oligofamily of chs_H1 is expressed in both generative and somatic tissues, suggesting a more universal promoter. Novel transcription factors (TFs) from bZIP and bHLH families involved in chs_H1 regulation have been found by means of genomic and cDNA libraries screening. These include HlbZIP1A and its truncated variant HlbZIP1B, HlbZIP2 and HlbHLH. The activation potential of HlbZIP2 and previously cloned hop TFs were analysed in P.hybrida and N.benthamiana transient expression systems. HlbZIP2, as well as both HlMyb1 and HlMyb3 were found as activators of chs_H1 promoter. In combinatorial system, HlbZIP2 was found to modulate the expression of both Myb TFs from hop.

Název v anglickém jazyce

Cloning and Molecular Analyses of Hop Transcription Factors

Popis výsledku anglicky

Expression of hop chs_H1 and other hop chalcon synthase (CHS)-like enzymes was assayed in developing pollen tissue. Both chs_H1 and valerophenone synthase (vps) are highly expressed in pollen suggesting the existence of common regulatory element(s). While vps is specific for female and male floral tissues, the oligofamily of chs_H1 is expressed in both generative and somatic tissues, suggesting a more universal promoter. Novel transcription factors (TFs) from bZIP and bHLH families involved in chs_H1 regulation have been found by means of genomic and cDNA libraries screening. These include HlbZIP1A and its truncated variant HlbZIP1B, HlbZIP2 and HlbHLH. The activation potential of HlbZIP2 and previously cloned hop TFs were analysed in P.hybrida and N.benthamiana transient expression systems. HlbZIP2, as well as both HlMyb1 and HlMyb3 were found as activators of chs_H1 promoter. In combinatorial system, HlbZIP2 was found to modulate the expression of both Myb TFs from hop.

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the Second International Humulus Symposium

ISBN

978-90-6605-722-7

ISSN

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e-ISSN

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Počet stran výsledku

8

Strana od-do

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Název nakladatele

ISHA Acta Horticulturae

Místo vydání

Ghent

Místo konání akce

Ghent

Datum konání akce

1. 9. 2009

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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