Cloning and Molecular Analysis of HlbZip1 and HlbZip2 Transcription Factors Putatively Involved in the Regulation of the Lupulin Metabolome in Hop (Humulus lupulus L.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F10%3A00340099" target="_blank" >RIV/60077344:_____/10:00340099 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/14864347:_____/10:#0000303 RIV/60076658:12310/10:00011808

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cloning and Molecular Analysis of HlbZip1 and HlbZip2 Transcription Factors Putatively Involved in the Regulation of the Lupulin Metabolome in Hop (Humulus lupulus L.)

Popis výsledku v původním jazyce

TFs HlbZIP1A and HlbZIP2 cloned from a cDNA library based on glandular tissue-enriched hop cones are similar to the group A3 bZIP TFs according to characteristic domains. While HlbZIP1A is rather neutral (pl 6.42), HlbZIP2 is strongly basic (pl 8,51). Both bZIP TFs show specific expression in lupulin glands, although low expression was observed also in roots and immature pollen. In a transient expression system, both hop bZIP TFs activated the GUS-coupled promoters of chalcone synthase chs_H1 and O-methyl transferase 1, while the response of the valerophenone synthase promoter to the cloned hop TFs was very low, showing that the cloned bZIP TFs are not strictly G-box-specific. HPLC analysis of secondary metabolites in infiltrated Petunia hybrida showedthat both bZIPs interfere with the accumulation and the composition of flavonol glycosides, phenolic acids, and anthocyanins, suggesting the possibility of coregulating flavonoid biosynthetic pathways in hop glandular tissue.

Název v anglickém jazyce

Cloning and Molecular Analysis of HlbZip1 and HlbZip2 Transcription Factors Putatively Involved in the Regulation of the Lupulin Metabolome in Hop (Humulus lupulus L.)

Popis výsledku anglicky

TFs HlbZIP1A and HlbZIP2 cloned from a cDNA library based on glandular tissue-enriched hop cones are similar to the group A3 bZIP TFs according to characteristic domains. While HlbZIP1A is rather neutral (pl 6.42), HlbZIP2 is strongly basic (pl 8,51). Both bZIP TFs show specific expression in lupulin glands, although low expression was observed also in roots and immature pollen. In a transient expression system, both hop bZIP TFs activated the GUS-coupled promoters of chalcone synthase chs_H1 and O-methyl transferase 1, while the response of the valerophenone synthase promoter to the cloned hop TFs was very low, showing that the cloned bZIP TFs are not strictly G-box-specific. HPLC analysis of secondary metabolites in infiltrated Petunia hybrida showedthat both bZIPs interfere with the accumulation and the composition of flavonol glycosides, phenolic acids, and anthocyanins, suggesting the possibility of coregulating flavonoid biosynthetic pathways in hop glandular tissue.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Agricultural and Food Chemistry

ISSN

0021-8561

e-ISSN

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Svazek periodika

58

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

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Kód UT WoS článku

000273671900032

EID výsledku v databázi Scopus

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