Cloning and molecular analyses of hop transcription regulation factors.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F14864347%3A_____%2F09%3A%230000230" target="_blank" >RIV/14864347:_____/09:#0000230 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cloning and molecular analyses of hop transcription regulation factors.

Popis výsledku v původním jazyce

To identify novel transcription factors involved in the regulation of chs_H1 genes in lupulin glands, cone-specific ESTs showing homology to bZIP and bHLH were utilized to screen hop cDNA and genomic libraries. Additionally, inverse PCR was used to amplify full length cDNAs of members of two bZIP oligofamilies from hop. Transcription factors HlbZIP1A and HlbZIP2 have predicted molecular masses of 27.4 and 34.2 kDa, respectively. While HlbZIP1A is rather neutral (pI 6.42), HlbZIP2 is strongly basic (pI 8.51). A truncated variant of HlbZIP1 (HlbZIP1B) that is strongly basic but lacks the zipper domain was also cloned from hop. Additionally, the first bHLH transcription factor was cloned from hop. HlbHLH1 is a protein of 33.6 kDa with a predicted pI of 6.08. Based on semiquantitative RT PCR results, this TF is expressed specifically in hop floral and cone tissue.

Název v anglickém jazyce

Cloning and molecular analyses of hop transcription regulation factors.

Popis výsledku anglicky

To identify novel transcription factors involved in the regulation of chs_H1 genes in lupulin glands, cone-specific ESTs showing homology to bZIP and bHLH were utilized to screen hop cDNA and genomic libraries. Additionally, inverse PCR was used to amplify full length cDNAs of members of two bZIP oligofamilies from hop. Transcription factors HlbZIP1A and HlbZIP2 have predicted molecular masses of 27.4 and 34.2 kDa, respectively. While HlbZIP1A is rather neutral (pI 6.42), HlbZIP2 is strongly basic (pI 8.51). A truncated variant of HlbZIP1 (HlbZIP1B) that is strongly basic but lacks the zipper domain was also cloned from hop. Additionally, the first bHLH transcription factor was cloned from hop. HlbHLH1 is a protein of 33.6 kDa with a predicted pI of 6.08. Based on semiquantitative RT PCR results, this TF is expressed specifically in hop floral and cone tissue.

Druh

C - Kapitola v odborné knize

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Proceedings of the Second International Humulus Symposium

ISBN

9789066057227

Počet stran výsledku

8

Strana od-do

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Počet stran knihy

321

Název nakladatele

International Society for Horticultural Science

Místo vydání

Leuven, Belgie

Kód UT WoS kapitoly

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