Plant Ribonucleases and Nucleases as Antiproliferative Agens Targeting Human Tumors Growing in Mice

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F10%3A00340102" target="_blank" >RIV/60077344:_____/10:00340102 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985904:_____/10:00340102

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Plant Ribonucleases and Nucleases as Antiproliferative Agens Targeting Human Tumors Growing in Mice

Popis výsledku v původním jazyce

Recombinant plant nucleases of tomato (TBN1) and hop (HBN1) (submitted to patenting under no. PV 2008-384;Z7585) were isolated to homogeneity and examined for their antitumor effects and cytotoxicity. Although antiproliferative effects of both recombinant nucleases were not significant on the ML-2 cell culture in vitro, the nucleases were strongly cytostatic in vivo after their administration intravenously as stabilized conjugates with polyethylene glycol (PEG). However, the antitumor effect of the first nuclease tested, the mung bean nuclease (PhA), on ML2 human tumor cell line was neither effective, even when significant antitumor activity was detected on human melanoma tumors in vivo. Recombinant TBN1 and HBN1 were as effective against human melanoma tumors as previously studied pine pollen nuclease and PhA and their effects were reached at about ten times lower concentrations compared to the use of bovine seminal RNase (BS-RNase).

Název v anglickém jazyce

Plant Ribonucleases and Nucleases as Antiproliferative Agens Targeting Human Tumors Growing in Mice

Popis výsledku anglicky

Recombinant plant nucleases of tomato (TBN1) and hop (HBN1) (submitted to patenting under no. PV 2008-384;Z7585) were isolated to homogeneity and examined for their antitumor effects and cytotoxicity. Although antiproliferative effects of both recombinant nucleases were not significant on the ML-2 cell culture in vitro, the nucleases were strongly cytostatic in vivo after their administration intravenously as stabilized conjugates with polyethylene glycol (PEG). However, the antitumor effect of the first nuclease tested, the mung bean nuclease (PhA), on ML2 human tumor cell line was neither effective, even when significant antitumor activity was detected on human melanoma tumors in vivo. Recombinant TBN1 and HBN1 were as effective against human melanoma tumors as previously studied pine pollen nuclease and PhA and their effects were reached at about ten times lower concentrations compared to the use of bovine seminal RNase (BS-RNase).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Recent Patents on DNA & Gene Sequences

ISSN

1872-2156

e-ISSN

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Svazek periodika

4

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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