Association of aster yellows subgroup 16SrI-C phytoplasmas with a disease of Ribes rubrum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00367360" target="_blank" >RIV/60077344:_____/11:00367360 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Association of aster yellows subgroup 16SrI-C phytoplasmas with a disease of Ribes rubrum

Popis výsledku v původním jazyce

A Ribes rubrum plant showing malformation and twisting of branches was found in a garden in South Bohemia. Ultrathin sections of tissues from symptomatic shoots revealed the presence of phytoplasma-like bodies. Different primer sets were used for amplification of the 16S-23S ribosomal gene segment. RFLP analysis and sequencing for phytoplasma identification classified the detected phytoplasma in the aster yellows group, subgroup 16SrI-C. Successful transmission of detected phytoplasma by dodder to periwinkle was confirmed by detection of numerous phytoplasma bodies in ultrathin sections of C. roseus and by PCR from dodder and periwinkle. RFLP analyses of PCR products as well as nucleotide sequences of the currant plant and symptomatic periwinkles wereidentical. Sequenced data obtained from both currant and indicator plant, were aligned and sequences of 1,613 bp were found to be identical. Transmissions of phytoplasma by grafting to healthy currant rootstocks were unsuccessful.

Název v anglickém jazyce

Association of aster yellows subgroup 16SrI-C phytoplasmas with a disease of Ribes rubrum

Popis výsledku anglicky

A Ribes rubrum plant showing malformation and twisting of branches was found in a garden in South Bohemia. Ultrathin sections of tissues from symptomatic shoots revealed the presence of phytoplasma-like bodies. Different primer sets were used for amplification of the 16S-23S ribosomal gene segment. RFLP analysis and sequencing for phytoplasma identification classified the detected phytoplasma in the aster yellows group, subgroup 16SrI-C. Successful transmission of detected phytoplasma by dodder to periwinkle was confirmed by detection of numerous phytoplasma bodies in ultrathin sections of C. roseus and by PCR from dodder and periwinkle. RFLP analyses of PCR products as well as nucleotide sequences of the currant plant and symptomatic periwinkles wereidentical. Sequenced data obtained from both currant and indicator plant, were aligned and sequences of 1,613 bp were found to be identical. Transmissions of phytoplasma by grafting to healthy currant rootstocks were unsuccessful.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/QH91224" target="_blank" >QH91224: Výzkum metod ozdravování rybízu.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Bulletin of Insectology

ISSN

1721-8861

e-ISSN

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Svazek periodika

64

Číslo periodika v rámci svazku

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Stát vydavatele periodika

IT - Italská republika

Počet stran výsledku

2

Strana od-do

65-66

Kód UT WoS článku

000296681900030

EID výsledku v databázi Scopus

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