Phytoplasma: Methods and Protocols. Dodder Transmission of Phytoplasmas

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00425832" target="_blank" >RIV/60077344:_____/13:00425832 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Phytoplasma: Methods and Protocols. Dodder Transmission of Phytoplasmas

Popis výsledku v původním jazyce

Transmission of phytoplasmas from naturally infected plant host species using the parasitic plant Cuscuta spp. to Catharanthus roseus is an effective way to maintain a wide range of phytoplasmas for further research. Here, transmission via dodder from aninfected plant Rehmannia glutinosa var. purpurea and from a symptomatic red currant plant (Ribes spp.) to C. roseus indicator plants using a ?stable bridges method is described. Typical symptoms of phytoplasma disease on periwinkle plants were obtained:virescent flowers with an increased number of axillary shoots and smaller leaves on periwinkle plants after transmission from R. purpurea, and greening flower petals (virescence) after transmission from Ribes spp. Phytoplasmas could be detected in donorand recipient plants by electron microscopy and by PCR assays using universal phytoplasma primer pairs. RFLP analyses of PCR fragments can also be used to confirm the identity of the phytoplasmas from donor and recipient plants.

Název v anglickém jazyce

Phytoplasma: Methods and Protocols. Dodder Transmission of Phytoplasmas

Popis výsledku anglicky

Transmission of phytoplasmas from naturally infected plant host species using the parasitic plant Cuscuta spp. to Catharanthus roseus is an effective way to maintain a wide range of phytoplasmas for further research. Here, transmission via dodder from aninfected plant Rehmannia glutinosa var. purpurea and from a symptomatic red currant plant (Ribes spp.) to C. roseus indicator plants using a ?stable bridges method is described. Typical symptoms of phytoplasma disease on periwinkle plants were obtained:virescent flowers with an increased number of axillary shoots and smaller leaves on periwinkle plants after transmission from R. purpurea, and greening flower petals (virescence) after transmission from Ribes spp. Phytoplasmas could be detected in donorand recipient plants by electron microscopy and by PCR assays using universal phytoplasma primer pairs. RFLP analyses of PCR fragments can also be used to confirm the identity of the phytoplasmas from donor and recipient plants.

Druh

O - Ostatní výsledky

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/QH91224" target="_blank" >QH91224: Výzkum metod ozdravování rybízu.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů