Conversion of the chill susceptible fruit fly larva (Drosophila melanogaster) to a freeze tolerant organism

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00375752" target="_blank" >RIV/60077344:_____/12:00375752 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/12:43883594

Výsledek na webu

<a href="http://www.pnas.org/content/early/2012/02/06/1119986109.full.pdf+html" target="_blank" >http://www.pnas.org/content/early/2012/02/06/1119986109.full.pdf+html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1073/pnas.1119986109" target="_blank" >10.1073/pnas.1119986109</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Conversion of the chill susceptible fruit fly larva (Drosophila melanogaster) to a freeze tolerant organism

Popis výsledku v původním jazyce

This paper shows that mechanisms of freeze-tolerance studied by us previously in subarctic fly Chymomyza costata can be applied to a fruit fly of tropical origin with a weak innate capacity to tolerate even mild chilling. We found that surprisingly simple laboratory manipulations can change the chill susceptible insect to the freeze-tolerant one. Larvae of Drosophila melanogaster can then survive at subzero temperatures when approximately 50% of their body water turns to ice crystals. To achieve this goal, synergy of two fundamental prerequisites is required: (a) shutdown of larval development, including all the chill sensitive processes linked to it, by exposing larvae to low but above-lethal temperatures (quiescence), and (b) incorporating the free amino acid proline in tissues by feeding larvae a proline-augmented diet (cryopreservation).

Název v anglickém jazyce

Conversion of the chill susceptible fruit fly larva (Drosophila melanogaster) to a freeze tolerant organism

Popis výsledku anglicky

This paper shows that mechanisms of freeze-tolerance studied by us previously in subarctic fly Chymomyza costata can be applied to a fruit fly of tropical origin with a weak innate capacity to tolerate even mild chilling. We found that surprisingly simple laboratory manipulations can change the chill susceptible insect to the freeze-tolerant one. Larvae of Drosophila melanogaster can then survive at subzero temperatures when approximately 50% of their body water turns to ice crystals. To achieve this goal, synergy of two fundamental prerequisites is required: (a) shutdown of larval development, including all the chill sensitive processes linked to it, by exposing larvae to low but above-lethal temperatures (quiescence), and (b) incorporating the free amino acid proline in tissues by feeding larvae a proline-augmented diet (cryopreservation).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Proceedings of the National Academy of Sciences of the United States of America

ISSN

0027-8424

e-ISSN

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Svazek periodika

109

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

3270-3274

Kód UT WoS článku

000300828200023

EID výsledku v databázi Scopus

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