In situ derivatization-liquid liquid extraction as a sample preparation strategy for the determination of urinary biomarker prolyl-4-hydroxyproline by liquid chromatography-tandem mass spectrometry
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00375767" target="_blank" >RIV/60077344:_____/12:00375767 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/60461373:22330/12:43894048 RIV/00843989:_____/12:00102350
Výsledek na webu
<a href="http://onlinelibrary.wiley.com/doi/10.1002/jms.2952/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jms.2952/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jms.2952" target="_blank" >10.1002/jms.2952</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
In situ derivatization-liquid liquid extraction as a sample preparation strategy for the determination of urinary biomarker prolyl-4-hydroxyproline by liquid chromatography-tandem mass spectrometry
Popis výsledku v původním jazyce
The derivatization with alkyl chloroformates proceeds smoothly in aqueous media, and the desired reaction products are efficiently separated from interfering ionic components by their extraction into a water-immiscible organic phase. Here, this approachwas examined in detail for analysis of a potential urinary dipeptide biomarker L-prolyl-4-L-hydroxyproline (PHP) by liquid chromatography coupled to electrospray mass spectrometry. PHP was treated with a series of alkyl and fluoroalkyl chloroformates. The N-isobutyloxycarbonyl isobutyl ester derivative afforded a highest detector response from all the derivatized forms examined, including the nonderivatized PHP. The LC-MS/MS method was validated using a heptadeutero-PHP and PHP thus determined in humanurine in less than 5 min. The in-situ derivatization-liquid liquid extraction has been demonstrated to be a useful sample preparation strategy for the analysis of polar metabolites by LC-MS/MS in complex urine matrix.
Název v anglickém jazyce
In situ derivatization-liquid liquid extraction as a sample preparation strategy for the determination of urinary biomarker prolyl-4-hydroxyproline by liquid chromatography-tandem mass spectrometry
Popis výsledku anglicky
The derivatization with alkyl chloroformates proceeds smoothly in aqueous media, and the desired reaction products are efficiently separated from interfering ionic components by their extraction into a water-immiscible organic phase. Here, this approachwas examined in detail for analysis of a potential urinary dipeptide biomarker L-prolyl-4-L-hydroxyproline (PHP) by liquid chromatography coupled to electrospray mass spectrometry. PHP was treated with a series of alkyl and fluoroalkyl chloroformates. The N-isobutyloxycarbonyl isobutyl ester derivative afforded a highest detector response from all the derivatized forms examined, including the nonderivatized PHP. The LC-MS/MS method was validated using a heptadeutero-PHP and PHP thus determined in humanurine in less than 5 min. The in-situ derivatization-liquid liquid extraction has been demonstrated to be a useful sample preparation strategy for the analysis of polar metabolites by LC-MS/MS in complex urine matrix.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Mass Spectrometry
ISSN
1076-5174
e-ISSN
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Svazek periodika
47
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
9
Strana od-do
294-302
Kód UT WoS článku
000301575200004
EID výsledku v databázi Scopus
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