Heptafluorobutyl chloroformate-based sample preparation protocol for nonchiral and chiral amino acid analysis by gas chromatography-mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00510309" target="_blank" >RIV/60077344:_____/19:00510309 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-1-4939-9639-1_18" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-9639-1_18</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-9639-1_18" target="_blank" >10.1007/978-1-4939-9639-1_18</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Heptafluorobutyl chloroformate-based sample preparation protocol for nonchiral and chiral amino acid analysis by gas chromatography-mass spectrometry

Popis výsledku v původním jazyce

Gas chromatography (GC) is a commonly used technique in amino acid analysis (AAA). However, one of the requirements of the application of GC for AAA is a need for the polar analytes to be converted into their volatile, thermally stable derivatives. In the last two decades, alkyl chloroformates (RCFs) have become attractive derivatization reagents. The reagents react immediately with most amino acid functional groups in aqueous matrices, and the process can easily be coupled with liquid-liquid extraction of the resulting less polar derivatives into immiscible organic phase. Here we describe a simple protocol for in situ derivatization of amino acids with heptafluorobutyl chloroformate (HFBCF) followed by subsequent chiral as well as nonchiral GC/MS (mass spectrometric) analysis on a respective nonpolar fused silica and an enantioselective Chirasil-Val capillary column.

Název v anglickém jazyce

Heptafluorobutyl chloroformate-based sample preparation protocol for nonchiral and chiral amino acid analysis by gas chromatography-mass spectrometry

Popis výsledku anglicky

Gas chromatography (GC) is a commonly used technique in amino acid analysis (AAA). However, one of the requirements of the application of GC for AAA is a need for the polar analytes to be converted into their volatile, thermally stable derivatives. In the last two decades, alkyl chloroformates (RCFs) have become attractive derivatization reagents. The reagents react immediately with most amino acid functional groups in aqueous matrices, and the process can easily be coupled with liquid-liquid extraction of the resulting less polar derivatives into immiscible organic phase. Here we describe a simple protocol for in situ derivatization of amino acids with heptafluorobutyl chloroformate (HFBCF) followed by subsequent chiral as well as nonchiral GC/MS (mass spectrometric) analysis on a respective nonpolar fused silica and an enantioselective Chirasil-Val capillary column.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA17-22276S" target="_blank" >GA17-22276S: Nové metody pro metabolomickou analýzu obtížně stanovitelných metabolitů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Amino Acid Analysis: Methods and Protocols

ISBN

978-1-4939-9638-4

Počet stran výsledku

15

Strana od-do

237-251

Počet stran knihy

455

Název nakladatele

Springer Science+Business Media, LLC, part of Springer Nature 2019

Místo vydání

New York

Kód UT WoS kapitoly

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