Differential response of Drosophila cell lines to extracellular adenosine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00376113" target="_blank" >RIV/60077344:_____/12:00376113 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/12:43883567

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0965174812000033" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0965174812000033</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ibmb.2012.01.002" target="_blank" >10.1016/j.ibmb.2012.01.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Differential response of Drosophila cell lines to extracellular adenosine

Popis výsledku v původním jazyce

Adenosine (Ado) is a crucial metabolite that influences proliferation of several vertebrate and invertebrate cells. Key proteins regulating Ado signaling, transport and metabolism are conserved among vertebrates and invertebrates. Here we show that Ado negatively influences viability, changes morphology and mitochondrial polarity of the Drosophila imaginal disc cell line (Cl.8ţ) via a mechanism exclusively dependent on cellular Ado uptake. High transport of Ado is followed by ATP production as a part ofAdo salvation, which at higher concentrations may interfere with cellular homeostasis. In contrast, hematopoietic cell line Mbn2, which grows well in high Ado concentration, preferentially uses adenosine deaminase as a part of the purine catabolic pathway. Our results show that different types of Drosophila cell lines use different pathways for Ado conversion and suggest that such differences may be an important part of complex mechanisms maintaining energy homeostasis in the body.

Název v anglickém jazyce

Differential response of Drosophila cell lines to extracellular adenosine

Popis výsledku anglicky

Adenosine (Ado) is a crucial metabolite that influences proliferation of several vertebrate and invertebrate cells. Key proteins regulating Ado signaling, transport and metabolism are conserved among vertebrates and invertebrates. Here we show that Ado negatively influences viability, changes morphology and mitochondrial polarity of the Drosophila imaginal disc cell line (Cl.8ţ) via a mechanism exclusively dependent on cellular Ado uptake. High transport of Ado is followed by ATP production as a part ofAdo salvation, which at higher concentrations may interfere with cellular homeostasis. In contrast, hematopoietic cell line Mbn2, which grows well in high Ado concentration, preferentially uses adenosine deaminase as a part of the purine catabolic pathway. Our results show that different types of Drosophila cell lines use different pathways for Ado conversion and suggest that such differences may be an important part of complex mechanisms maintaining energy homeostasis in the body.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Insect Biochemistry and Molecular Biology

ISSN

0965-1748

e-ISSN

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Svazek periodika

42

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

321-331

Kód UT WoS článku

000302515800002

EID výsledku v databázi Scopus

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