Disparate phenotypic effects from the knockdown of various Trypanosoma brucei cytochrome c oxidase subunits

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00377492" target="_blank" >RIV/60077344:_____/12:00377492 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/12:43883429

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0166685112001065#" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0166685112001065#</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molbiopara.2012.04.013" target="_blank" >10.1016/j.molbiopara.2012.04.013</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Disparate phenotypic effects from the knockdown of various Trypanosoma brucei cytochrome c oxidase subunits

Popis výsledku v původním jazyce

The Trypanosoma brucei cytochrome c oxidase (respiratory complex IV) is a very divergent complex containing a surprisingly high number of trypanosomatid-specific subunits with unknown function. To gain insight into the functional organization of this large protein complex, the expression of three novel subunits were down-regulated by RNA interference. We demonstrated that all three subunits were important for the structural integrity of the cytochrome c oxidase complex and their knockdowns caused severephenotypes related to mitochondrial functions. Surprisingly, these subunits produced a disparate effect regarding the activity of the neighboring complex III and ROS production. These results suggest that all three subunits are genuine subunits of T. brucei complex IV, though they may have different functional roles, perhaps involving the ability to communicate between sequential enzymes in the respiratory chain.

Název v anglickém jazyce

Disparate phenotypic effects from the knockdown of various Trypanosoma brucei cytochrome c oxidase subunits

Popis výsledku anglicky

The Trypanosoma brucei cytochrome c oxidase (respiratory complex IV) is a very divergent complex containing a surprisingly high number of trypanosomatid-specific subunits with unknown function. To gain insight into the functional organization of this large protein complex, the expression of three novel subunits were down-regulated by RNA interference. We demonstrated that all three subunits were important for the structural integrity of the cytochrome c oxidase complex and their knockdowns caused severephenotypes related to mitochondrial functions. Surprisingly, these subunits produced a disparate effect regarding the activity of the neighboring complex III and ROS production. These results suggest that all three subunits are genuine subunits of T. brucei complex IV, though they may have different functional roles, perhaps involving the ability to communicate between sequential enzymes in the respiratory chain.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and Biochemical Parasitology

ISSN

0166-6851

e-ISSN

—

Svazek periodika

184

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

90-98

Kód UT WoS článku

000306626300004

EID výsledku v databázi Scopus

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