Eliminace jaderně kódovaných podjednotek komplexů III a IV způsobuje rozpad těchto komplexů ale ne komplexu I u Trypanosoma brucei

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077409%3A_____%2F05%3A00023757" target="_blank" >RIV/60077409:_____/05:00023757 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/05:00006342

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Downregulation of the nuclear-encoded subunits of the complexes III and IV disrupts their respective complexes but not complex I in procyclic Trypanosoma brucei

Popis výsledku v původním jazyce

The function, stability and mutual interactions of selected nuclear-encoded subunits of respiratory complexes III and IV were studied in the Trypanosoma brucei procyclics using RNA interference (RNAi). Western analysis of mitochondrial lysates using specific antibodies confirmed complete elimination of the targeted proteins 4 to 6 days post-induction. As revealed by two-dimensional blue native/SDS PAGE electrophoresis, silencing of a single subunit resulted in the disruption of the respective complex, while the other complex remained unaffected. Membrane potential was reproducibly decreased in the knock-downs and the activities of complex III and/or IV, but not complex I, were drastically reduced, as measured by activity assays and histochemical staining. The apparent absence in T. brucei procyclics of a supercomplex composed of complexes I and III may represent an ancestral state of the respiratory chain.

Název v anglickém jazyce

Downregulation of the nuclear-encoded subunits of the complexes III and IV disrupts their respective complexes but not complex I in procyclic Trypanosoma brucei

Popis výsledku anglicky

The function, stability and mutual interactions of selected nuclear-encoded subunits of respiratory complexes III and IV were studied in the Trypanosoma brucei procyclics using RNA interference (RNAi). Western analysis of mitochondrial lysates using specific antibodies confirmed complete elimination of the targeted proteins 4 to 6 days post-induction. As revealed by two-dimensional blue native/SDS PAGE electrophoresis, silencing of a single subunit resulted in the disruption of the respective complex, while the other complex remained unaffected. Membrane potential was reproducibly decreased in the knock-downs and the activities of complex III and/or IV, but not complex I, were drastically reduced, as measured by activity assays and histochemical staining. The apparent absence in T. brucei procyclics of a supercomplex composed of complexes I and III may represent an ancestral state of the respiratory chain.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/IAA5022302" target="_blank" >IAA5022302: Funkční analýza vybraných proteinů účastnících se editování RNA u bičíkovce Trypanosoma brucei</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Microbiology

ISSN

0950-382X

e-ISSN

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Svazek periodika

58

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

15

Strana od-do

116-130

Kód UT WoS článku

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EID výsledku v databázi Scopus

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